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RNA-seq of retinal microglia and non-microglial retina cells in WT (Bmal1f/f) and microglial Bmal1-KO (Bmal1-mg/-mg) mice

ABSTRACT: We discovered that Bmal1 knockdown in retinal microglia alters their diurnal clock gene expression, inflammatory gene expression, and morphology. Furthermore, we found that this disruption of diurnal physiology in microglia results in reduced scotopic visual function, retinal neurodegeneration, and abnormal wheel running behavior in mice, suggesting that maintenance of the microglial clock contributes to retinal homeostasis. Subsequently, we investigated transcriptional alterations in retinal microglia and non-microglial cells in the retina to uncover gene expression patterns that may explain these clock-dependent phenotypes and potentially identify homeostatic neuro-glial signaling mechanisms dependent on microglial clock biology.

ORGANISM(S): Mus musculus

PROVIDER: GSE282175 | GEO | 2025/07/28

REPOSITORIES: GEO

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Microglial gene expression in Bmal1 cKO and WT mice

Project description:To investigate the interaction between aging and microglial circadian rhythmicity, we examined mice deficient in the core clock transcription factor, BMAL1, in microglia.

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RNA-seq of retinal microglia and non-microglial retina cells in WT (Bmal1f/f) and microglial Bmal1-KO (Bmal1-mg/-mg) mice

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Microglial REV-ERBα regulates inflammation and lipid droplet formation to drive tauopathy

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Physical exercise improves the neuronal function in ischemic stroke via microglial CB2R/P2Y12 signaling

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Microglial activation protects from vascular degeneration in a rat model of inflammatory retinal vasoregression.

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Microglial proteomic signatures in APPPS1 and APP-KI mice

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Transcription profiling time series of the response of human microglial cells to interferon gamma

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