Single Cell Profiling of Chromatin Accessibility in Cotton Ovule Epidermis Durring Early Fiber Development [scATAC-seq]
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ABSTRACT:
ORGANISM(S): Gossypium hirsutum
PROVIDER: GSE282999 | GEO | 2025/12/02
REPOSITORIES: GEO
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Project description:Each cotton seed has approximately 25,000 singular fiber cells, which account for 25-30% epidermal cells. Understanding the basis of fiber cell initiation is critical for cotton yield improvement. However, it is difficult to separate and study fiber and non-fiber cells. Here we developed a reliable single-cell protocol to study transcriptome changes in Upland and Pima cotton and in a naked seed mutant. We analyzed over 40,000 single cells derived predominately from the epidermal layer of ovules during early stages of fiber development.
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