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RNA sequencing of neutrophils treated with microvesicles derived from glucose-deprived hepatocellular carcinoma cells.

ABSTRACT: Microvesicles derived from glucose-deprived liver cancer cells can promote tumor initiation and progression through neutrophils. This phenomenon can be rescued by knocking down Ezrin. Comparative analysis revealed that microvesicles from Ezrin-knockdown (Ezrin KD) liver cancer cells exhibit downregulation of VEGFA and PD-L1, along with upregulation of angiogenesis and T-cell regulatory pathways.

ORGANISM(S): Mus musculus

PROVIDER: GSE283439 | GEO | 2026/04/01

REPOSITORIES: GEO

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hepatocellular carcinoma after glucose-free microvesicle treatment

Project description:As tumor progression occurs, liver cancer cells are often in a state of nutrient deprivation, particularly lacking glucose. We found that microvesicles secreted by liver cancer cells under glucose-free conditions can promote tumorigenesis and progression. The specific mechanism involves enhancing neutrophil expression of PD-L1 and VEGFA, thereby suppressing T cell cytotoxicity and promoting angiogenesis.This phenotype is abolished after Ezrin knockout in liver cancer cells.

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RNA sequencing of neutrophils treated with microvesicles derived from glucose-deprived hepatocellular carcinoma cells.

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