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22

SMAD3 ChIP-on-chip on A549 cell line


ABSTRACT: The aim of this study is to identify SMAD3 binding targets affected by the TGFb1/SMAD3 signal transduction on a genome-wide scale Overall design: Formaldehyde cross-linked, sonicated chromatin was prepared from non-stimulated A549 cell line and stimulated with TGFb1. Chromatin immunoprecipated with anti-SMAD3 antibody is labeled with Cy5 and mock IP (with anti-FLAG antibody) is labeled with Cy3 and co-hybridized on Agilent Human Promoter Set arrays. Two biological replicates were performed.

INSTRUMENT(S): Agilent-014706 Human Promoter ChIP-on-Chip Set 244K, Microarray 1 of 2 G4489A (Probe name version)

ORGANISM(S): Homo sapiens  

SUBMITTER: Kusum Vijay Pandit   

PROVIDER: GSE28346 | GEO | 2011-06-01

SECONDARY ACCESSION(S): PRJNA139453

REPOSITORIES: GEO

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