Transcriptomics

Dataset Information

25

Protection from obesity and diabetes by blockade of TGF-beta/Smad3 signaling


ABSTRACT: Imbalances in glucose and energy homeostasis are at the core of the worldwide epidemic of obesity and diabetes. Here, we illustrate an important role of the TGF-beta/Smad3 signaling pathway in regulating glucose and energy homeostasis. Smad3 deficient mice are protected from diet-induced obesity and diabetes. Interestingly, the metabolic protection is accompanied by Smad3-/- white adipose tissue acquiring the bioenergetic and gene expression profile of brown fat/skeletal muscle. Smad3-/- adipocytes demonstrate a marked increase in mitochondrial biogenesis, with a corresponding increase in basal respiration, and Smad3 acts as a repressor of PGC-alpha1 expression. We observe significant correlation between TGF-beta1 levels and adiposity in rodents and humans. Further, systemic blockade of TGF-beta1 signaling protects mice from obesity, diabetes and hepatic steatosis. Together, these results demonstrate that TGF-beta signaling regulates glucose tolerance and energy homeostasis and suggest that modulation of TGF-beta1 activity might be an effective treatment strategy for obesity and diabetes. Overall design: Smad3-/- and WT mice were fed with regular diet (RD) and high fat diet (HFD), and diet induced obese (DIO) mice were treated with IgG and anti-TGF-b1 antibody

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus  

SUBMITTER: WeiPing Chen  

PROVIDER: GSE28598 | GEO | 2011-07-13

SECONDARY ACCESSION(S): PRJNA139107

REPOSITORIES: GEO

Similar Datasets

2011-07-12 | E-GEOD-28598 | ArrayExpress
| GSE69913 | GEO
2012-04-12 | E-GEOD-36032 | ArrayExpress
2015-06-17 | E-GEOD-69913 | ArrayExpress
2015-06-18 | E-GEOD-68317 | ArrayExpress
2016-08-25 | E-MTAB-4989 | ArrayExpress
2008-06-14 | E-GEOD-5777 | ArrayExpress
| GSE104956 | GEO
| GSE95489 | GEO
2018-07-09 | GSE104029 | GEO