Monitoring of functional gene responses to biostimulation from a TCE-contaminated site
Ontology highlight
ABSTRACT:
ORGANISM(S): Pseudomonas Nitrosomonas Sulfurospirillum Shewanella Desulfitobacterium Dehalobacter Methylosinus Mycobacterium Thauera groundwater metagenome Geobacter Dehalococcoides
PROVIDER: GSE28608 | GEO | 2012/04/03
SECONDARY ACCESSION(S): PRJNA143087
REPOSITORIES: GEO
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Project description:A functional microarray targeting 24 genes involved in chlorinated solvent biodegradation pathways has been developed and used to monitor the gene expression in a contaminated site (site B) under ERD (enhanced reductive dechlorination) treatment. The microarray format provided by NimbleGen and used in this study is 12x135K. 4 M-BM-5g of labelled antisense mRNA from 3 groundwater samples were hybridized on the microarray. A 3-chip study was performed, each corresponding to hybridization with 4 M-BM-5g of labelled antisense mRNA retrieved from a monitoring well of a contaminated site (site B). Each probe (760nt) on the microarray was synthesized in eight replicates, and a total of 5,707 random probes was used to determine the background noise. Groundwater samples were collected from a contaminated site (site B) from three monitoring wells (P1, P2 and P3). P1: well located upstream to the contamination source. P2: well in the contamination source. P3 : well located downstream to the contamination source.
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Project description:This SuperSeries is composed of the following subset Series: GSE28606: Monitoring of functional gene responses to ERD (enhanced reductive dechlorination) from four TCE-contaminated sites GSE28608: Monitoring of functional gene responses to biostimulation from a TCE-contaminated site Refer to individual Series
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