Rroid2 regulates effector and memory CD8+ T cell response during infection in vivo

ABSTRACT: CD8+ T cell differentiation has been associated with changes in the expression of long noncoding RNA (lncRNAs). Yet, which and how lncRNAs regulate CD8+ T cell responses following infection in vivo remain incompletely understood. We performed deep RNA-seq to map the lncRNA expression landscape of CD8+ T cell subsets during infection and generated lncRNA knockout mouse models to evaluate the in vivo relevance of six lncRNAs. We identified the enhancer lncRNA Rroid2 to regulate effector CD8+ T cell function as well as effector-to-memory differentiation. Rroid2-deficient mice displayed increased CD44dim Foxp3+ regulatory T cells while the development of other immune cells, such as natural killer cells, was not affected. In CD8+ T cells, Rroid2 deficiency resulted in a fine-tuned downregulation of transcription factors Id2 and T-bet and impaired effector CD8+ T cell proliferation and cytotoxicity as well as memory CD8+ T cell generation and recall responses. In contrast to Id2, Rroid2 deficiency affected both KLRG1+ and KLRG1- effector CD8+ T cells. Taken together, Rroid2 represents a key regulatory layer that controls CD8+ T cell responses to pathogens.

ORGANISM(S): Mus musculus

PROVIDER: GSE286902 | GEO | 2025/12/09

REPOSITORIES: GEO