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Phosphate starvation induces root cell-type specific transcriptional responses and alternative splicing

ABSTRACT: Optimal plant nutrition is crucial for plant growth, development, and production. Phosphorus (P) is a vital macronutrient for plants, but its availability in soil is often limited. Despite high levels of P in natural soils, much of it is inaccessible to plants. Plants acquire phosphorus as inorganic phosphate (Pi) from the rhizosphere, and Pi starvation triggers adaptive responses to enhance Pi acquisition. These responses include changes in root development and gene transcriptional reprogramming among others. Although several root transcriptomic analyses have identified key genes involved in Pi starvation responses, almost all of them have been conducted with illuminated roots, a condition that masks molecular responses to Pi starvation. In this work, a comparative transcriptomic analysis of specific root cell types identified over 7,000 genes with differential expression in response to Pi deficiency. Additionally, this study highlights the role of alternative splicing in regulating Pi starvation responses and demonstrates that both transcriptional and posttranscriptional responses are cell type specific. Overall, this work provides new insights into the cell-specific responses to Pi starvation, enhancing our understanding of plant adaptation to phosphorus deficiency.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE287184 | GEO | 2026/03/12

REPOSITORIES: GEO

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