Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DRG bulk RNAseq: Repeated Injections Of Intra-articular Nerve Growth Factor Into The Naive Murine Knee Joint Cause Synovial Inflammation, Knee Sensitization, And Neuronal Sprouting

ABSTRACT: We aimed to explore the pain-producing effects of repeated intra-articular (IA) injections of NGF into the knee joints of healthy mice, as well as the effects on joint innervation and structure. In addition DRG bulk RNAseq was performed.

ORGANISM(S): Mus musculus

PROVIDER: GSE287526 | GEO | 2025/07/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

DRG bulk RNAseq: Repeated Injections Of Intra-articular Nerve Growth Factor Into The Naive Murine Knee Joint Cause Synovial Inflammation, Knee Sensitization, And Neuronal Sprouting

Project description:DRG bulk RNAseq: Repeated Injections Of Intra-articular Nerve Growth Factor Into The Naive Murine Knee Joint Cause Synovial Inflammation, Knee Sensitization, And Neuronal Sprouting

| PRJNA1213144 | ENA

Expression data from the rat knee articular cartilage at different developmental stages

Project description:Age as the primary rise factor could be play an important role in incidence and development of osteoarthritis. Several studies have confirmed some tissue specific microRNA were associated with development of osteoarthritis. But if age related microRNA or miRNA cluster would be involved in pivotal post-transcriptional gene regulation in osteoarthritis is unclear. In view of this, we have an idea that several age-related miRNAs would be screened from the rat knee cartilage at different development ages by miRNAs Microarray analysis. We used microarrays to detail the global programme of gene expression underlying the rat knee cartilage and identified distinct classes of age-related miRNAs during this process. The rat knee articular cartilage were selected at successive stages of the rat developmental for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of cartilage at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected cartilage according to the rat developmental stages, i.e. seven time-points: newborn (T0), childhood (T1), youth(T2), adult (T3), middle-aged (T4) early-stage elderly(T5) and latter-stage elderly(T6). The objective of the study is to identify miRNA profile of knee articular cartilage at different developmental ages in rats. Total RNA were extracted from the knee articular cartilage of Sprague-Dawley rats at postnatal day 0(T0), week1(T1), week 4(T2), mon3(T3), mon 6(T4), mon 12(T5), and mon 18(T6). The microRNA profile in the specimens was detected with the Affymetrix GeneChipÂ® miRNA 3.0 Array.

2013-06-26 | E-GEOD-48266 | biostudies-arrayexpress

Gene expression analyses in mouse sensory ganglia determine a crucial role of NGF/TrkA in knee osteoarthritis chronification

Project description:Objective: Osteoarthritis (OA) can be experimentally induced by injecting mono-iodoacetate (MIA) in the knee capsule of mice. Our aim was to assess the role of NGF/TrkA axis in OA identifying differentially expressed genes (DEGs) and functional pathways in knee-innervating dorsal root ganglia (DRG) from WT and hypersensitive TrkAP782S (KI) mice after MIA injection. Method: We performed saline or MIA-injection in knee joints of WT and KI mice and harvested L3-L5 DRGs at 5 and 21 days after injection, pooling males and females (n = 4/group). RNA was extracted and microarray analysis was performed. Upon comparisons between different groups, identification of DEGs was defined as adjusted P < 0.01. Gene ontology, pathway analysis and protein interactions were conducted using Gene Set Enrichment Analysis over Gene Ontology and REACTOME databases, and STRING database. Results: For each comparison regarding genotype (WT vs KI), numerous DEGs were identified but with limited overlap, being Lingo1, Socs2, and Slc4a4 already related to pain, OA and/or NGF/TrkA axis. Regarding comparisons of early vs late OA (D5 vs D21), many more DEGs were revealed including genes previously implicated in OA such as Gal, Gja1, and Lep. Moreover, we found enriched pathways in the KI_MIA group such as gene expression, neuronal system and signal transduction, in which NTRK1 and MAPK pathways indicate specificity in the NGF/TrkA axis and in the transition from early to late OA pain. Conclusions: Our results identify new mouse DEGs and pathways that demonstrate the relevance of the NGF/TrkA system in the chronification of OA pain.

2025-06-18 | GSE291152 | GEO

Intra-articular knee injections: procedures and assessments video series.

Project description: Not available

| S-EPMC3625085 | biostudies-literature

Expression data from the rat knee articular cartilage at different developmental stages

2013-06-26 | GSE48266 | GEO

Repeated intra-articular injections of umbilical cord-derived mesenchymal stem cells for knee osteoarthritis: a phase I, single-arm study.

Project description: Not available

| S-EPMC10262357 | biostudies-literature

Biocompatible Solutions: Evaluating the Safety of Repeated Intra-Articular Injections of pMPCylated Liposomes for Knee Osteoarthritis Therapy in Rat Models.

Project description: Not available

| S-EPMC11414135 | biostudies-literature

Expression data of LncRNA from the rat (Sprague-Dawley) knee articular cartilage at different developmental stages

Project description:Age as the primary rise factor could be play an important role in incidence and development of osteoarthritis. A few studies have confirmed some tissue specific lncRNA were associated with development of osteoarthritis. But if age related lncRNA would be involved in pivotal post-transcriptional gene regulation in osteoarthritis is unclear. In view of this, we have an idea that several age-related lncRNA would be screened from the rat knee cartilage at different development ages by lncRNAs Microarray analysis. We used microarrays to detail the global programme of gene expression underlying the rat knee cartilage and identified distinct classes of age-related lncRNA during this process. The rat knee articular cartilage were selected at successive stages of the rat developmental for RNA extraction and hybridization on Affymetrix lncRNA arrays. We sought to obtain homogeneous populations of cartilage at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we hand-selected cartilage according to the rat developmental stages, i.e. seven time-points: newborn (T0), youth(T1), adult (T2), early-stage elderly(T3) and latter-stage elderly(T4).

2015-03-05 | E-GEOD-66554 | biostudies-arrayexpress

Intra-articular platelet-rich plasma injections versus intra-articular corticosteroid injections for symptomatic management of knee osteoarthritis: systematic review and meta-analysis.

Project description: Not available

| S-EPMC8208610 | biostudies-literature

Osteoarthritis-related changes in gene expression in knee articular chondrocytes in a rat model of osteoarthritis.

Project description:Cartilage destruction in osteoarthritis (OA) results from disturbed chondrocyte metabolism. Here, we used microarrays to show that TGF alpha and CCL2 are simultaneously upregulated in a rat model of OA and cooperate to drive cartilage degradation. The goals of the experiments included here were to a) characterize gene expression in knee joint articular chondrocytes at various stages of development of OA (2 and 8 weeks after surgical induction of OA), and b) to establish trends in gene expression among groups of genes related to the TGF alpha-EGFR axis, over time, in OA. The model chosen to study these results has been previously validated (Appleton, CT et al, 2007, Arthritis Rheum) and used to describe similar gene expression results at a different time point (4 weeks) after induction of OA. The rat model of OA involves surgical destabilization of the knee joint, followed by forced low-intensity mobilization over several weeks; a sham surgery is used as the control (representing a healthy non-OA knee joint) wherein a surgical incision is made but not structural (i.e. ligamentous) modification is made to the joint. Altogether, our data indicate that TGF and CCL2 cooperate to drive cartilage degradation in osteoarthritis. A total of 12 samples were analyzed. 3 replicates were used per condition: OA surgery at 2 weeks, OA surgery at 8 weeks, Sham (control) surgery at 2 weeks and Sham surgery at 8 weeks. Expression of OA samples was assessed relaitve to Sham (control) expression levels.

2012-11-15 | E-GEOD-42295 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data