Mechanistic basis of gene regulation by SRCAP and H2A.Z (CUT&RUN)

ABSTRACT: Discriminating regulatory functions of chromatin composition from those of chromatin-modifying complexes is a central problem in gene regulation. This question remains unexplored in the context of histone variants and their dedicated chromatin remodelers. Here we dissect the distinct and cell cycle-dependent functions of Snf2 Related CREBBP Activator Protein (SRCAP) and H2A.Z in gene regulation of pluripotent stem cells. Using acute degradation of endogenous SRCAP, we uncover dynamic changes of H2A.Z occupancy and continuous requirement of SRCAP over the cell cycle. We also engineered an SRCAP mutant, defective for H2A.Z deposition, allowing us to distinguish H2A.Z-dependent and independent functions of SRCAP. We discover that SRCAP exhibits essential H2A.Z-independent functions in inhibiting DNA binding of dozens of pioneer transcription factors at enhancers by steric hindrance. In contrast, H2A.Z acts mainly as a transcriptional repressor gatekeeping the expression of lineage-specific genes. Our study establishes the catalytic-independent role of a chromatin remodeler in broadly regulating transcription factor binding, and demonstrates how a chromatin remodeler-histone variant pair orchestrates transcription to maintain self-renewal and plasticity of pluripotent stem cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE287553 | GEO | 2026/01/06

REPOSITORIES: GEO