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CRISPLD2 regulates adipocyte function

ABSTRACT: Obesity with accompanying adipose fibrosis is associated with worsening insulin resistance. Weight loss and adipose tissue turnover are conversly associated with improved insulin signaling and overall health. We previously found the secreted protein CRISPLD2 up-regulated in adipocytes during weight loss; CRISPLD has been suggested regulate inflammation and tissue turnover. As loss of CRISPLD2 is embryonic lethal, we developed mice with adipocyte-specific inducible CRISPLD2 overexpression to explore its function. Single-cell RNAseq analysis of the stromal vascular fraction (SVF) during long-term adipocyte CRISPLD2 overexpression demonstrated broad down-regulation of collagen transcription across multiple cell types; we found similar down-regulation of fibrosis via picosirius red staining of adipose tissue overexpressing CRISPLD2, along with a decrease in adipocyte cell size in chow-fed animals.

ORGANISM(S): Mus musculus

PROVIDER: GSE287885 | GEO | 2026/01/01

REPOSITORIES: GEO

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