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Proper nuclear compartment reorganization in G1 phase is essential for DNA replication control [scRepli-seq]

ABSTRACT: Upon G1 entry after mitosis, mammalian chromosomal DNA is spatially segregated into A and B compartments, which correlate well with classic euchromatin and heterochromatin, respectively. The significance of this spatial segregation, however, has never been explored for lack of means to manipulate this level of chromosomal organization. Through a genome-wide CRISPR screen, we identify GINS4, a component of the replicative DNA helicase complex, as an essential factor for A/B compartment segregation in G1. By manipulating the G1 compartment reorganization process through GINS4, we show that it is required for efficient DNA synthesis and proper DNA replication timing (RT) regulation in the subsequent S-phase. Our findings reveal an unexpected role of GINS4 and highlight the significance of nuclear compartmentalization in DNA replication control.

ORGANISM(S): Mus musculus

PROVIDER: GSE287969 | GEO | 2026/06/12

REPOSITORIES: GEO

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Proper nuclear compartment reorganization in G1 phase is essential for DNA replication control [Hi-C]

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