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Prostaglandin and prostacyclin regulation of Th1 responses [ptgir_ko]

ABSTRACT: Metabolic reprogramming is an integral component of normal Th1 induction and contraction. Whereas Th1 inducing signals are largely defined, the pathways underlying timely Th1 contraction remain poorly understood. Here, we show that a CD4+ T cell-intrinsic prostanoid lipid mediator production and signaling machinery, controlled by cell-autonomous complement C5, governs the Th1 shutdown program.

ORGANISM(S): Mus musculus

PROVIDER: GSE288921 | GEO | 2025/06/12

REPOSITORIES: GEO

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Prostaglandin and prostacyclin regulation of Th1 responses [ptgir_ptgis_sirna]

Project description:Metabolic reprogramming is an integral component of normal Th1 induction and contraction. Whereas Th1 inducing signals are largely defined, the pathways underlying timely Th1 contraction remain poorly understood. Here, we show that a CD4+ T cell-intrinsic prostanoid lipid mediator production and signaling machinery, controlled by cell-autonomous complement C5, governs the Th1 shutdown program.

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Project description:T helper cell activation is highly regulated to ensure proper immune responses while avoiding autoimmune reactions. Cell functions are determined by regulation of various levels of gene expression including posttranslational modifications of proteins. Protein prenylation is a posttranslational modification, which can be influenced by statin treatment and was shown to play a role in the differentiation and activation of various T cell populations, including Th1 cells. However, it is largely unknown which proteins are prenylated in Th1 cells and what the effect of statin treatment on protein prenylation is in this context. Here, using mass spectrometry, we show the comprehensive identification of prenylated proteins in human Th1 cells including changes during their activation, before analyzing the effect of statin treatment on this process. This approach additionally uncovered the prenylation of proteins, not yet known to be prenylated, including so far unknown motifs. These data provide valuable insights into novel aspects of protein prenylation in Th1 cell activation and deepen our understanding of the intended or unintended effects of statin treatment on the human immune system.

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