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Impact of Acute methionine restriction on proteome of Th1 cells - Part 2

ABSTRACT: CD4 T cells were activated using CD3/CD28 antibodies in the presence of IL2 and IL12, to generate Th1 cells. Th1 cells were maintained and expanded in IL2 and IL12, on D5 Th1 cells were sorted for CD4+ expression and DAP1 exclusion. After flow sorting, live TH1 cells were resuspended (1e6 per ml) in methionine free RPMI, supplemented with 10% dialysed FBS, IL2 and IL12, and with L-methionine (100μM, or 1μM). Cells were cultured for 5 hrs before collection for proteomics processing.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): T Cell, Lymph Node

SUBMITTER: Andrew Howden

LAB HEAD: Professor Doreen Cantrell

PROVIDER: PXD012053 | Pride | 2019-03-29

REPOSITORIES: Pride

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Antigen receptor control of methionine metabolism in T cells.

Sinclair Linda V LV Howden Andrew Jm AJ Brenes Alejandro A Spinelli Laura L Hukelmann Jens L JL Macintyre Andrew N AN Liu Xiaojing X Thomson Sarah S Taylor Peter M PM Rathmell Jeffrey C JC Locasale Jason W JW Lamond Angus I AI Cantrell Doreen A DA

eLife 20190327

Immune activated T lymphocytes modulate the activity of key metabolic pathways to support the transcriptional reprograming and reshaping of cell proteomes that permits effector T cell differentiation. The present study uses high resolution mass spectrometry and metabolic labelling to explore how murine T cells control the methionine cycle to produce methyl donors for protein and nucleotide methylations. We show that antigen receptor engagement controls flux through the methionine cycle and RNA a ...[more]

PMID: 30916644

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