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Integrated Transcriptomics and Metabolomics Analyses Revealed that Phenylpropanoid Metabolism Regulates the Complex Salt-alkali Tolerance in Allium mongolicum

ABSTRACT: Allium mongolicum, a xerophytic industrial plant native to the Mongolian Plateau, demonstrates notable stress resistance, though its salt-alkali tolerance mechanisms remain poorly understood. This study integrates physiological assessments, transcriptomics, metabolomics, and full-length transcriptome analyses to uncover its adaptive mechanisms under salt-alkali stress.A comprehensive full-length transcriptome was constructed under these conditions, comprising 30,117 non-redundant genes, alongside significant trends observed in the activities of antioxidant enzymes and key enzymes in the phenylpropanoid pathway. Combined transcriptomic and metabolomic analyses identified key genes and metabolites in the phenylpropanoid pathway as central to salt-alkali tolerance. WGCNA further highlighted critical genes, such as AmCOMT1, AmHSP18, and AmPPL7, with functional validation of AmCOMT1 and AmHSP18 confirming transcriptome reliability. The salt tolerance function of AmCOMT1 has been validated through overexpression in plants, and the binding interaction with AmERF4 has also been confirmed.A proposed model suggests A. mongolicum mitigates salt-alkali stress via reactive oxygen species scavenging, osmotic regulation, and structural support, providing valuable insights for breeding salt-tolerant crops and enhancing its agricultural applications.

ORGANISM(S): Allium mongolicum

PROVIDER: GSE289259 | GEO | 2026/02/08

REPOSITORIES: GEO

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Integrated Transcriptomics and Metabolomics Analyses Revealed that Phenylpropanoid Metabolism Regulates the Complex Salt-alkali Tolerance in Allium mongolicum

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