Single-cell multi-omics provide insights into molecular drivers of CAR T Cell Persistence and Function in Multiple Myeloma

ABSTRACT: The underpinning mechanisms driving chimeric antigen receptor (CAR) T cell function and persistence after infusion, and their correlation with clinical outcome, remains poorly understood. Here, we applied single-cell multiomics, coupled with gene regulatory network analyses, to evaluate CAR-T cell transcriptional heterogeneity, dynamics and clonal evolution, in longitudinal bone marrow (BM) and peripheral blood (PB) paired samples, collected from MM patients treated with BCMA CAR T cells. We identify several regulons with increased activity, within CAR T cell infiltrating the BM, as key drivers of the more activated and exhausted phenotype observed in those cells compared to their PB counterparts. Interestingly, regulatory dysfunctions in the effector-to-memory axis promoted higher presence of terminally differentiated cells, leading to a lack of CAR T cell persistence. Moreover, we identified a hyperexpanded CAR T clone in the BM of a patient with partial response. Deeper characterization showed increased IL10 expression in that clone, that was associated to transcription factors related to exhausted CAR T cells, like PRDM1. Overall, our analyses provide insights into the regulatory mechanisms that could promote lack of CAR T cell persistence and dysfunction, representing potential targets to be modulated for the development of improved CAR T therapies for MM.

ORGANISM(S): Homo sapiens

PROVIDER: GSE290061 | GEO | 2026/03/04

REPOSITORIES: GEO