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Transcriptional differences between effector cells derived from freshly isolated and long-lived naïve P14 CD8 T cells on day 5 post-infection.

ABSTRACT: The heterogeneity of antigen-specific naïve CD8 T cells has not yet been fully elucidated. We found that long-lived naïve CD8 T cells, incubated in naïve B6 mice for more than 30 days, generate significantly higher numbers of effector and memory CD8 T cells after lymphocytic choriomeningitis virus (LCMV)-Armstrong infection compared to freshly isolated naive CD8 T cells. To examine the mechanism of why long-lived naive CD8 T cells exhibit superior responses to infection, we compared transcriptional profiles between effector CD8 T cells derived from long-lived and freshly isolated naive CD8 T cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE290460 | GEO | 2025/11/14

REPOSITORIES: GEO

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Transcriptional differences between freshly isolated and long-lived naïve P14 CD8 T cells prior to infection

Project description:The heterogeneity of antigen-specific naïve CD8 T cells has not yet been fully elucidated. We found that long-lived naïve CD8 T cells, incubated in naïve B6 mice for more than 30 days, generate significantly higher numbers of effector and memory CD8 T cells after lymphocytic choriomeningitis virus (LCMV)-Armstrong infection compared to freshly isolated naive CD8 T cells. To examine differences between long-lived and freshly isolated naive CD8 T cells prior to infection, a bulk RNA-seq analysis was performed.

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Transcriptional differences between freshly isolated and long-lived naïve P14 CD8 T cells prior to infection

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Expression data from LCMV-infected P14 and Akt transgenic P14 CD8 T cells

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Transcriptional differences between effector cells derived from freshly isolated and long-lived naïve P14 CD8 T cells on day 5 post-infection.

Project description:Transcriptional differences between effector cells derived from freshly isolated and long-lived naïve P14 CD8 T cells on day 5 post-infection.

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ATAC-seq of P14 CD8 T cells during acute LCMV infection

Project description:We performed RNA-seq, ATAC-seq, and H3K27Ac ChIP-seq on P14 CD8 T cells during acute infection with LCMV Armstrong on days 0 (naïve cells), 9 (memory precursor effector cells (MPEC) and short-lived effector cells (SLECs)), and 40+ (memory cells) post infection.

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RNA-seq of P14 CD8 T cells during acute LCMV infection

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H3K27Ac ChIP-seq of P14 CD8 T cells during acute LCMV infection

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miRNA signatures of antigen specific CD8+ T cells at different stages of immune response to LCMV infection

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