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A metabolism-chromatin axis promotes ribosome heterogeneity in the human malaria parasite

ABSTRACT: The transmission of the most virulent human malaria parasite, Plasmodium falciparum, relies on the survival in the contrasting environments of the human host and mosquito vector. One of the most fascinating adaptations to this lifestyle is the specific silencing of individual rDNA genes in the human host that are de-repressed following host-to-vector transmission. In this study, we characterized the epigenetic signatures of active and silent rDNA loci and found that rDNA silencing relies on aerobic glycolysis, the sole energy-generating pathway in the human host. We show that disruption of NAD+ regeneration during lactate fermentation promotes rDNA de-repression and identify Sir2 as the mediator between fluctuating NAD+ levels and a functional transcriptional outcome. Hence, rDNA activation appears to be coupled to the metabolic state of the parasite as it transitions from aerobic glycolysis to mitochondrial respiration during host-to-vector transmission.

ORGANISM(S): Plasmodium falciparum

PROVIDER: GSE290639 | GEO | 2025/11/25

REPOSITORIES: GEO

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A metabolism-chromatin axis promotes ribosome heterogeneity in the human malaria parasite [II]

Project description:The transmission of the most virulent human malaria parasite, Plasmodium falciparum, relies on the survival in the contrasting environments of the human host and mosquito vector. One of the most fascinating adaptations to this lifestyle is the specific silencing of individual rDNA genes in the human host that are de-repressed following host-to-vector transmission. In this study, we characterized the epigenetic signatures of active and silent rDNA loci and found that rDNA silencing relies on aerobic glycolysis, the sole energy-generating pathway in the human host. We show that disruption of NAD+ regeneration during lactate fermentation promotes rDNA de-repression and identify Sir2 as the mediator between fluctuating NAD+ levels and a functional transcriptional outcome. Hence, rDNA activation appears to be coupled to the metabolic state of the parasite as it transitions from aerobic glycolysis to mitochondrial respiration during host-to-vector transmission.

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