The role of Inmt knockout in renal proximal tubules.
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE291123 | GEO | 2025/12/04
REPOSITORIES: GEO
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Project description:Background. Chronic renal failure is characterized by progressive renal scarring and accelerated cardiovascular disease. In animal models, this is thought to be due to non-dialyzable uremic toxins – small, protein-bound molecules normally secreted via Organic Anion Transporters (OATs) in the proximal renal tubule,rather than filtered at the glomerulus. The best studied of these is indoxyl sulfate (IS). Methods. We examined global gene expression responses in cultured normal human renal tubular cells incubated with control plasma (n=5) or pre- and post-dialysis uremic plasma (n=10). Results. GeneSpring analysis of microarrays revealed significantly altered expression of 2016 genes. The expression of 537 genes “normalized” in post-dialysis plasma, suggesting removal of “low molecular weight uremic toxins” by dialysis. The expression of the majority of dysregulated genes (1479) was not normalized in post-dialysis plasma. These likely represent the effects of substances not effectively removed by dialysis (“protein-bound uremic toxins”). Addition of indoxyl sulfateto control plasma simulated most effects (81.1%) of uremic plasma, while the addition of probenecid, an OAT inhibitor, to uremic plasma reversed most changes in gene expression. Analysis of molecular programs with the Gene Set Enrichment Analysis and the DAVID database revealed patterns common to indoxyl sulfate-treated control plasma, pre-dialysis, and post-dialysis uremic plasma. These included increased cell cycle, pro-inflammatory, and pro-fibrotic molecular programs, particularly genes in the TGF-β pathway. Conclusion: These findings provide insight into the role of non-dialyzable, protein-bound uremic toxins in the pathogenesis of renal scarring and uremic vasculopathy. The GSEA patterns confirm that inflammatory and fibrotic programs are active.
2013-06-30 | GSE45709 | GEO
Project description:End-stage renal disease (ESRD) is the final stage of chronic kidney disease, which is increasingly prevalent worldwide and is associated with the progression of cardiovascular disease (CVD). Indoxyl sulfate (IS) and p-cresyl sulfate (PCS), major uremic toxins, are major risk factors involved in the pathology of CVD via adverse effects on endothelial cells and immune cells. Thus, transcriptomic overview of uremic toxin-mediated genes in immune cells of ESRD patients is critical, but not yet fully known. We investigated the alteration of gene expressions and biological pathways mediated by major uremic toxins, in ESRD patients monocytes, via microarray analysis. To explore uremic toxin-related transcriptional profiling in ESRD patient-derived monocytes, purified monocytes from healthy controls were treated with IS or PCS for 24 hr, followed by conducting microarray on these samples.
2020-07-30 | GSE155325 | GEO
Project description:The role of Inmt knockout in renal proximal tubules.
| PRJNA1232033 | ENA
Project description:Background. Chronic renal failure is characterized by progressive renal scarring and accelerated cardiovascular disease. In animal models, this is thought to be due to non-dialyzable uremic toxins M-bM-^@M-^S small, protein-bound molecules normally secreted via Organic Anion Transporters (OATs) in the proximal renal tubule,rather than filtered at the glomerulus. The best studied of these is indoxyl sulfate (IS). Methods. We examined global gene expression responses in cultured normal human renal tubular cells incubated with control plasma (n=5) or pre- and post-dialysis uremic plasma (n=10). Results. GeneSpring analysis of microarrays revealed significantly altered expression of 2016 genes. The expression of 537 genes M-bM-^@M-^\normalizedM-bM-^@M-^] in post-dialysis plasma, suggesting removal of M-bM-^@M-^\low molecular weight uremic toxinsM-bM-^@M-^] by dialysis. The expression of the majority of dysregulated genes (1479) was not normalized in post-dialysis plasma. These likely represent the effects of substances not effectively removed by dialysis (M-bM-^@M-^\protein-bound uremic toxinsM-bM-^@M-^]). Addition of indoxyl sulfateto control plasma simulated most effects (81.1%) of uremic plasma, while the addition of probenecid, an OAT inhibitor, to uremic plasma reversed most changes in gene expression. Analysis of molecular programs with the Gene Set Enrichment Analysis and the DAVID database revealed patterns common to indoxyl sulfate-treated control plasma, pre-dialysis, and post-dialysis uremic plasma. These included increased cell cycle, pro-inflammatory, and pro-fibrotic molecular programs, particularly genes in the TGF-M-NM-2 pathway. Conclusion: These findings provide insight into the role of non-dialyzable, protein-bound uremic toxins in the pathogenesis of renal scarring and uremic vasculopathy. The GSEA patterns confirm that inflammatory and fibrotic programs are active. Transcriptomic remodeling in response to external stimulus RNA from non-immortalized cultured renal cortical cells incubated with plasma samples from 5 controls, 10 uremics pre-dialysis, 10 uremics post-dialysis, 5 controls with indoxyl sulfate added, 5 uremics pre-dialysis with probenecid added, 5 uremics post-dialysis with indoxyl sulfate added
2013-06-30 | E-GEOD-45709 | biostudies-arrayexpress
Project description:Renal failure is associated with accumulation of various solutes called Uremic toxins. Post transcriptional regulation related to Chronic kidney disease (CKD) have already been described as RNA based silencing with micro RNA or modifications of mRNA degradation. Until now, alternative splice modification was not mentioned in the course of CKD. However, CKD is associated with modification of gene expression. The aim of the study was to explore modification of the alternative splice pattern in the course in CKD. The expression level of individual exons expression in human fibroblast were compared after culture to 96 hours of uremic condition or control condition. Three independant experiments were performed
2013-08-16 | E-GEOD-41030 | biostudies-arrayexpress
Project description:Transcriptome analysis of human coronary arterial endothelial cells (HCAEC) primary cultures exposed to uremic serum from patients with stage 4-5 cardiovascular diseases (CKD) without myocardial infarction (USI) and stage 4-5 CKD with myocardial infarction (UCI). Gene expression profile of human coronary arterial endothelial cells (HCAEC) primary cultures exposed to uremic serum from of patients with chronic kidney disease (CKD) that explore which pathways are involved in in the development of cardiovascular disease. The metabolic pathway significantly represented was the MAPK signaling pathway, suggesting that the effects of uremic toxins present in patients with CDK, on the primary cultures of HCAEC provides a more informative model for the study of molecular mechanisms that lead to the development of endothelial dysfunction secondary to CKD.
2019-01-31 | GSE125898 | GEO
Project description:Chronic kidney disease (CKD), defined as a glomerular filtration rate <60 mL/min/1.73 m2 for at least 3 months, is a major public health concern with high prevalence in developed countries. CKD patients present dysregulations in their coagulation system, with higher levels of factor VII, factor VIII, thrombin, and tissue factor (main initiator of blood coagulation in vivo). Paradoxically, these patients present both an increased risk of thrombotic events and a higher bleeding rate compared to healthy subjects. Traditional cardiovascular risk factors are insufficient to explain the elevated rates of CVD, since it is the leading cause of death among CKD patients, rising uremic toxins as a possible link. Uremic toxins are compounds eliminated by the kidneys in physiological conditions. They tend to accumulate in the blood and tissues of patients with CKD due to its inadequate renal clearance. Uremia could influence biotransformation and transport across cellular membranes, disturbing drug tissue distribution. Therefore, CKD not only alters the elimination of drugs excreted by the kidneys, but it also impacts the metabolism of drugs subject to non-renal clearance, which involves mainly the liver and the gut. The liver is a highly specialized tissue, involved in the synthesis of plasma proteins and production of clotting factors, it has a major role in drug metabolization. The liver is also the main producer of thrombopoietin (hormone that regulates the production of platelets). All these processes are thought to be highly disturbed in CKD patients. Hepatocytes express high levels of AhR, ligand-inducible transcription factor activated by by xeno- and endobiotics, that mediate the induction of various liver cytochrome P450 enzymes. Hence, liver AhR regulates both nutrient metabolism and xenobiotic detoxification. Tryptophan derived uremic toxins (TDUT), induce tissue factor expression via the aryl hydrocarbon receptor (AhR) pathway, explaining the connection between CKD uremic toxin accumulation and hemostatic disbalances. Indoxyl sulfate (IS) is the main TDUT involved in uremic syndrome and acts as an AhR agonist in human primary hepatocytes. Tryptophan is metabolize to indole in the gut, absorbed into blood circulation and further process by the liver creating IS. In patients with CKD, IS tends to accumulate since it is poorly removed via dialysis. IS induces vascular endothelium and hemostatic dysfunction impacting cardiovascular morbidity and mortality during CKD. IS being a nephrotoxic, prothrombotic, pro-oxidative and pro-inflammatory molecule is proposed as the responsible for thrombotic events and metabolic changes in CKD patients. To further investigate the role of AhR and IS in metabolism and coagulation we want to analyse 60 C57BL/6J mice liver samples. These samples come from 8 different experimental groups: 4 groups of male mice and 4 groups of female mice. Each subset consists of a wildtype group treated with KCl (control), a KO AhR group treated with KCl (control), a wildtype group treated with 0,1% IS and a KO AhR group treated with 0,1% IS. The number of animals per group ranges from 6 to 8. Mice were fed ad libitum during the whole animal trial which started at 10 weeks of life. Both KCl and IS where solubilized in 5% sucrose water and administer in the drinking water of the mice for 1 week, after which mice were sacrificed at 11 weeks of age. KCl was used as control substance since IS is used under its potassium salt form. Although in many studies the variable gender is not considered, since we though that there could be a difference in basal expression as well as a hormonal bias that could influence the results, we decided to create separate experimental groups for both male and females. Hence, the large number of samples that needed to be analysed. The liver samples collected, were snap frozen and have been stored at −80 °C until further analyses.
2025-05-07 | PXD053404 | Pride
Project description:Renal failure is associated with accumulation of various solutes called Uremic toxins. Post transcriptional regulation related to Chronic kidney disease (CKD) have already been described as RNA based silencing with micro RNA or modifications of mRNA degradation. Until now, alternative splice modification was not mentioned in the course of CKD. However, CKD is associated with modification of gene expression. The aim of the study was to explore modification of the alternative splice pattern in the course in CKD.
2013-08-16 | GSE41030 | GEO
Project description:To clarify the effects of cisplatin (cis-diamminedichloroplatinum II, CDDP) on the gene expression profiles in renal proximal tubules, microarray analyses were carried out using total RNA samples isolated from microdissected proximal tubules and whole kidneys. The molecular events underlying acute kidney injury (AKI) in the proximal tubules of rats with cisplatin-induced nephrotoxicity were successfully clarified with 17,000 transcripts. Renal proximal tubules were isolated under microscopy, and transcriptome data were collected with Rat Genome Survey Microarray® (Applied Biosystems)
2013-12-24 | E-GEOD-37133 | biostudies-arrayexpress