Dataset Information

Diversity and complexity of auditory representation in the hearing systems of Aedes aegypti mosquitoes

ABSTRACT: While low-frequency sounds have been reported to stimulate dispersal responses in male and female mosquitoes, only males show attraction to sound. Male attraction to female flight tones is important during courtship; however, groups of males show diverse responses to acoustic stimuli, suggesting auditory processing can vary drastically between the sexes and individual males. To investigate diversity in auditory representation within and between the sexes, we utilised molecular and functional analyses to explore Aedes aegypti mosquito auditory processing. We identified shared and dimorphic neurons connecting mosquito ears to the brains’ primary auditory processing center. Calcium imaging from this brain region facilitated definition of multiple neuronal clusters based on auditory stimulation responses. More clusters with greater complexity were identified in males than females, with these clusters highly differentiated amongst males. Transcriptomic and proteomic analyses found enrichment of ciliary-related factors in male ears compared to females, potentially underlying sexual dimorphisms in hearing systems.

ORGANISM(S): Aedes aegypti

PROVIDER: GSE291714 | GEO | 2025/05/12

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Hepatocellular carcinoma (HCC) is the most common liver cancer and a highly malignant tumor. The frequent activation of Ras signaling pathway and the male prevalence are the distinct characteristics of HCC though the underlying mechanisms remain elucidated. By exploring a mouse model of HCC induced by hepatocyte-specific expression of the Hras12V oncogene and showed significant male prevalence in hepatic tumorigenesis, we performed a high-throughput comparative proteomic analysis based on tandem-mass-tag (TMT) labeling combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) on the protein samples from hepatic tumor tissues (T) and peri-tumor tissues (P) of transgenic males and females and the corresponding normal liver tissues (W) of non-transgenic males and females. In total, 5733 proteins were confidently identified, of which 5193 proteins were quantified. Finally, 1344 differentially expressed proteins (DEPs) (quantified in all examined samples; 1.5 ≤ quantitative ratios ≤ 0.67; p ≤ 0.05) were selected for further analysis. Vertical comparison within W, P, T of males and females, respectively, showed that the numbers of DEPs in males were much higher than females. Bioinformatics analyses showed the common and unique cluster enriched items between sexes which indicates the common and gender disparity pathways towards HCC. Further expression change pattern analysis revealed HCC positive/negative-related and Ras oncogene positive/negative-related DEPs. Horizontal comparison between males and females showed that Ras oncogene gradually and significantly reduced the responses to sex hormones from hepatocytes to hepatoma cells and therefore shrunk their gender disparity, which may contribute to the cause of the loss of HCC clinical responses to the therapeutic approaches targeting sex hormone pathways. Additionally, FABP5 was found to be a more sensitive biomarker for clinical diagnose and prognosis prediction of HCC patients comparing to AFP. In conclusion, the present study firstly provided a comprehensive proteome profiles and novel insights in male bias hepatic tumorigenesis induced by H-ras12V oncogene.

Dataset Information

Diversity and complexity of auditory representation in the hearing systems of Aedes aegypti mosquitoes

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets