Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole genome CRISPR drop-out screen in THP-1 cells exposed to mebendazole

ABSTRACT: We previously reported that mebendazole is an effective anti-AML therapeutic and acts via induction of MYB degradation. The present experiemnt was performed in order to uncover pathways of potential resistance in AML cells to mebendazole exposure. A whole genome CRISPR drop-out screen was performed on THP-1 AML cells exposed to 1 micromolar mebendazole or DMSO in order to identify genes that decreased AML sensitivity to this drug.

ORGANISM(S): Homo sapiens

PROVIDER: GSE293063 | GEO | 2026/04/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Whole genome CRISPR drop-out screen in THP-1 cells exposed to mebendazole

Project description:Whole genome CRISPR drop-out screen in THP-1 cells exposed to mebendazole

| PRJNA1242722 | ENA

Expression analysis of Mebendazole treated THP-1 cells in three paired samples

Project description:Mebendazole treatment of MLL rearanged cell line THP-1

2017-03-14 | GSE96544 | GEO

Targeting the unwindosome by mebendazole is a vulnerability of chemoresistant hepatoblastoma

Project description:We identified mebendazole as a drug to circumvent chemoresistance in hepatoblastoma cell lines that are resistant to cisplatin. In order to identify the mechanistic basis of mebendazole function, we performed gene expression analysis of three mebendazole-treated hepatoblastoma cell lines.

2022-07-28 | GSE209824 | GEO

Mebendazole Inhibits Histoplasma capsulatum In Vitro Growth and Decreases Mitochondrion and Cytoskeleton Protein Levels

Project description:Histoplasmosis is a frequent mycosis in people living with HIV/AIDS and other immunocompromised hosts. Histoplasmosis has high rates of mortality in these patients if treatment is unsuccessful. Itraconazole and amphotericin B are used to treat histoplasmosis; however, both antifungals have potentially severe pharmacokinetic drug interactions and toxicity. The present study determined the minimal inhibitory and fungicidal concentrations of mebendazole, a drug present in the NIH Clinical Collection, to establish whether it has fungicidal or fungistatic activity against Histoplasma capsulatum. Protein extracts from H. capsulatum yeasts, treated or not with mebendazole, were analyzed by proteomics to understand the metabolic changes driven by this benzimidazole. Mebendazole inhibited the growth of 10 H. capsulatum strains, presenting minimal inhibitory concentrations ranging from 5.0 to 0.08 µM. Proteomics revealed 30 and 18 proteins exclusively detected in untreated and mebendazole-treated H. capsulatum yeast cells, respectively. Proteins related to the tricarboxylic acid cycle, cytoskeleton, and ribosomes were highly abundant in untreated cells. Proteins related to the nitrogen, sulfur, and pyrimidine metabolisms were enriched in mebendazole-treated cells. Furthermore, mebendazole was able to inhibit the oxidative metabolism, disrupt the cytoskeleton, and decrease ribosomal proteins in H. capsulatum. These results suggest mebendazole as a drug to be repurposed for histoplasmosis treatment.

2023-03-22 | PXD033762 | Pride

miRNAs in resveratrol-treated THP-1 cells

Project description:We studied the role of microRNAs specifically upregulated in THP-1 cells following a 14 hours resveratrol treatment (50 micromolar).

2011-10-28 | E-TABM-1086 | biostudies-arrayexpress

miRNA profiing of human THP-1 cells treated with resveratrol

Project description:We studied the role of microRNAs specifically upregulated in THP-1 cells following a 14 hours resveratrol treatment (50 micromolar).

2010-03-01 | E-TABM-782 | biostudies-arrayexpress

Determining the DNA-binding targets of NFATc2 in the AML cell line THP-1

Project description:Based on previous work on the KDM histone demethylase family in AML, the transcription factor NFATC2 was identified as a regulatory target of KDM4A in MLL-AF9-rearrangement THP-1 cells. Our existing work examined the elements of the transcriptome regulated by NFATC2 in THP-1 cells and so, to further this work, we aimed to characterise the DNA-binding targets of NFATc2 in these cells. In this study, we used NFATc2 immunoprecipitation for ChIP-seq in untreated THP-1 cells, in order to determine these.

2024-09-01 | GSE241260 | GEO

MicroRNA profiling of human THP-1 cells following 14 hours of resveratrol treatment

Project description:We studied the role of microRNAs specifically upregulated in THP-1 cells following a 14 hours resveratrol treatment (50 micromolar).

2010-02-28 | E-TABM-780 | biostudies-arrayexpress

Mebendazole as Adjuvant Treatment for Colon Cancer

Project description:mebendazole treating colon cancer

| 2303689 | ecrin-mdr-crc

The transcriptomic response to albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), oxfendazole (OxBZ) and fenbendazole (FBZ) in the C. elegans strain CB3474 ben-1(e1880)III

Project description:We have undertaken a detailed analysis of the biotransformation of five of the most therapeutically important benzimidazole anthelmintics - albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), oxfendazole (OxBZ) and fenbendazole (FBZ) - in Caenorhabditis elegans. As a step to identifying nematode enzymes potentially responsible for benzimidazole biotransformation, we characterised the transcriptomic response to each of the benzimidazole drugs using the C. elegans strain CB3474 ben-1(e1880)III in order to minimize general phenotypic and stress responses to the drug. In the case of albendazole (ABZ), mebendazole (MBZ), thiabendazole (TBZ), and oxfendazole (OxBZ) - the shared transcriptomic response was dominated by the up- regulation classical xenobiotic response genes.

2019-04-30 | GSE130466 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data