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Natural Microbial exposure populates the maternal fetal interface with diverse T cells.

ABSTRACT: scRNAseq was used to investigate how maternal microbial burden influences the immunology of the maternal fetal interface (MFI). Female mice werre cohoused with pet store mice for >4 weeks prior to conception and throughout gestation to naturalize maternal immunity. Cohoused dams were compared to specific pathogen free (SPF) control dams. Anti-CD45.2 IV labeling techniques were used to delineate immune cells in tissues from those in contact with circulation. IV labeling also permitted enrichment of placental (IV+) and decidal (IV-) tissue associated immune cells postprocessing. This is the first scRNAseq dataset to compare the immune cell populations from the MFI (combined placental and decidual tissue) of SPF and 'dirty' (pet store cohoused) dams. These data revealed significant changes to the immune cell composition and expansion of populations of T cells that phenocopy those described in the human decidua.

ORGANISM(S): Mus musculus

PROVIDER: GSE293451 | GEO | 2025/07/30

REPOSITORIES: GEO

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