Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA interactions of transcriptional co-regulator SEUSS (SEU) during Arabidopsis flower development.

ABSTRACT: Transcriptional co-regulators are key modulators of gene expression, often functioning in complexes with transcription factors to regulate chromatin dynamics. LEUNIG_HOMOLOG (LUH) is a member of the conserved Groucho/Tup1 family of co-regulators in Arabidopsis, yet its role in flower development remains poorly understood. While LEUNIG (LUG), a homolog of LUH, has been extensively studied in floral organ identity specification, contributions of LUH appear to be both overlapping and distinct from LUG. Other co-regulators, such as SEUSS (SEU) and its homologs SEUSS-LIKE1-3 (SLK1-3), function as bridge proteins that stabilize the interactions between transcription factors and cofactors from the LUG/LUH family. Using high-throughput DNA occupancy analyses, we identify SEU target genes during flower development in Arabidopsis. Our findings contribute to understanding how LUH modulates transcriptional responses and chromatin state to fine-tune flower development in Arabidopsis.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE294839 | GEO | 2025/11/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome profiling of leunig_homolog (luh) and leunig (lug) mutants in floral induction system (FIS) during early stages of Arabidopsis flower development

Project description:Transcriptional co-regulators are key modulators of gene expression, often functioning in complexes with transcription factors to regulate chromatin dynamics. LEUNIG_HOMOLOG (LUH) is a member of the conserved Groucho/Tup1 family of co-regulators in Arabidopsis, yet its role in flower development remains poorly understood. While LEUNIG (LUG), a homolog of LUH, has been extensively studied in floral organ identity specification, contributions of LUH appear to be both overlapping and distinct from LUG. Here, we investigate the LUH regulome, uncovering its dynamic protein interactions with transcriptional cofactors, histone-modifying enzymes, chromatin remodelers, and transcription factors, including MADS-domain proteins APETALA1 and SEPALLATA3. Using high-throughput DNA occupancy and transcriptomic analyses, we identify LUH target genes and their expression dynamics across floral developmental stages. Comparative transcriptome profiling of luh and lug mutants in floral induction system reveals unique regulatory functions of LUH. Furthermore, we provide some evidence for a dual role of LUH in transcriptional activation and repression. Our findings contribute to understanding how LUH modulates transcriptional responses and chromatin state to fine-tune flower development in Arabidopsis.

2025-11-25 | GSE289681 | GEO

DNA interactions of transcriptional co-regulator LEUNIG_HOMOLOG (LUH) during early stages of Arabidopsis flower development.

2025-11-25 | GSE289372 | GEO

Expression data from inflorescence (unopened flower buds) of Arabidopsis thaliana - comparison between Ler and ask1 mutant

Project description:ARABIDOPSIS SKP1-LIKE1 (ASK1) protein is involved in regulating flower development. We have compared ask1 mutant floral transcriptome with wild-type Ler to identify the role of ASK1-containing E3 ubiquitin ligases in regulating flower transcriptome. In this dataset, we include the expression data obtained from Arabidopsis thaliana Ler and ask1 mutant flower buds. We identified 42 genes and 74 genes that are down-regulated and up-regulated, respectively.

2013-02-01 | GSE42841 | GEO

DNA interactions of transcriptional co-regulator SEUSS (SEU) during Arabidopsis flower development.

Project description:DNA interactions of transcriptional co-regulator SEUSS (SEU) during Arabidopsis flower development.

| PRJNA1251669 | ENA

Gene expression in luh mutant seeds in the dark: wild type (Col-0) vs. luh mutant

Project description:Transcriptional profiling of Arabidopsis far-red light pulse treated seeds comparing luh mutant with wild type (Col-0). Seeds were imbibed within 1 hr under white light and treated far-red light pulse for 5 min followed by 12 hr dark incubation. Goal was to determine the effects of LUH as transcriptional co-regulator during seed germination process.

2015-09-27 | GSE70406 | GEO

In-vitro Co-immunoprecipitation Mass Spectrometry of LUH and AP1 from Arabidopsis

Project description:This dataset contains quantitative co-immunoprecipitation mass spectrometry (Co-IP-MS) data generated to investigate direct interactions between the Arabidopsis co-regulator LUH and the MADS-domain transcription factor AP1. LUH wild type and domain variants (LUH_W, LUH_QW, LUH_LQ) were co-expressed with AP1 or empty vector controls, and proteins were immunoprecipitated using AP1-specific antibodies. Input and eluate fractions were analyzed by LC-MS/MS and quantified using MaxQuant. The results show that LUH interacts directly with AP1 via its C-terminal domains, supporting a distinct mode of co-regulator-MADS TF association.

2025-11-25 | PXD068765 | Pride

Expression data from inflorescence (unopened flower buds) of Arabidopsis thaliana - comparison between Ler and ask1 mutant

2013-02-01 | E-GEOD-42841 | biostudies-arrayexpress

DNA interactions of transcriptional co-regulator LEUNIG_HOMOLOG (LUH) during early stages of Arabidopsis flower development.

Project description:DNA interactions of transcriptional co-regulator LEUNIG_HOMOLOG (LUH) during early stages of Arabidopsis flower development.

| PRJNA1222793 | ENA

Protein interactions of transcriptional co-regulator LEUNIG_HOMOLOG (LUH) during early stages of Arabidopsis flower development.

Project description:To study the formation and dynamics of LUH protein-protein interactions at various stages of flower development, we conducted a series of immunoprecipitation followed by mass spectrometry (IP-MS) experiments using the LUH-GFP in floral induction system (FIS) plant line (pLUH:LUH-GFP pAP1:AP1-GR ap1 cal). We focused on four time points after the induction of flowering corresponding to: undifferentiated inflorescence meristem (0 days after induction, 0 DAI), meristem specification (2 DAI), organ specification (4 DAI), and organ differentiation (8 DAI).

2025-11-25 | PXD060520 | Pride

Transcriptomic characterization of a synergistic genetic interaction during carpel margin meristem development in Arabidopsis thaliana

Project description:We are interested in examining the transcriptional hierarchies that are required for proper formation and function of the carpel margin meristem in Arabidopsis thaliana. The carpel margin meristem (CMM) gives rise to the ovule primorida (which begin to form around flower stage 8). We know that the loss of the seuss-3 (seu-3) and aintegumenta-1(ant-1) separately leads to a mild disruption of ovule formation, while the loss of both causes a complete loss of ovule formation. In this study we wanted to examine expression levels changed between each single mutant and the double mutant. We dissected out stage 8-10 carpel of each of the four genotypes (seu-3, ant-1, Col-0, seu-3 ant-1) pooling 15-25 carpels into one biological replicate. Then using 16 ATH1 arrays we hybridized, amplified and labeled cDNA to 16 arrays (4 replicates for each genotype). We then analyzed our data to reveal transcripts that went down in the double compared to either single mutant, and transcripts that went down more than additively in the double mutant compared to added level of the decrease of the single mutant levels.

2011-08-01 | GSE30492 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data