Proteomics

Dataset Information

0

In-vitro Co-immunoprecipitation Mass Spectrometry of LUH and AP1 from Arabidopsis

ABSTRACT: This dataset contains quantitative co-immunoprecipitation mass spectrometry (Co-IP-MS) data generated to investigate direct interactions between the Arabidopsis co-regulator LUH and the MADS-domain transcription factor AP1. LUH wild type and domain variants (LUH_W, LUH_QW, LUH_LQ) were co-expressed with AP1 or empty vector controls, and proteins were immunoprecipitated using AP1-specific antibodies. Input and eluate fractions were analyzed by LC-MS/MS and quantified using MaxQuant. The results show that LUH interacts directly with AP1 via its C-terminal domains, supporting a distinct mode of co-regulator-MADS TF association.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) Triticum Aestivum (wheat)

SUBMITTER: Cezary Smaczniak  

LAB HEAD: Kerstin Kaufmann

PROVIDER: PXD068765 | Pride | 2025-11-25

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
20221110_KoRe_LUH-CDS_AP1_IP.raw Raw
20221110_KoRe_LUH-CDS_AP1_Input.raw Raw
20221110_KoRe_LUH-CDS_Empty_IP.raw Raw
20221110_KoRe_LUH-CDS_Empty_Input.raw Raw
20221110_KoRe_LUH-LQ_AP1_IP.raw Raw
Items per page:
1 - 5 of 18
altmetric image

Publications

Rewiring of LEUNIG_HOMOLOG interaction networks marks regulatory shifts from meristem to organ growth in Arabidopsis flowers.

Vega-León Rosario R   Muino Jose M JM   Zhu Tao T   Gluza Joanna A JA   Pimentel Maurizio M   Rehbein Konstantin K   Steiner Saoirse S   Rezzolla Emilia E   Golz John F JF   Chen Dijun D   Kaufmann Kerstin K   Smaczniak Cezary C  

The Plant journal : for cell and molecular biology 20251101 4

LEUNIG (LUG) and LEUNIG_HOMOLOG (LUH) are Groucho/Tup1-type transcriptional co-regulators in Arabidopsis thaliana that act redundantly across multiple developmental and environmental response pathways. Their specific contributions to flower development, however, have remained unclear due to embryonic lethality of double mutants. Here, we show that LUH associates with distinct sets of transcription factors and chromatin-associated proteins in a developmentally dynamic manner. Chromatin occupancy  ...[more]

Similar Datasets

Proteomics SPOROCYTELESS/NOZZLE interacts with MADS-DOMAIN transcription factors during the megaspore mother cell differentiation in the Arabidopsis ovule
2025-11-18 | PXD070409 | Pride
Proteomics Protein interaction partners of FRUITFULL in inflorescence meristems and pistils by IP-MS.
2023-03-30 | PXD037091 | Pride
Proteomics Protein interaction partner characterization of the tetramerization deficient APETALA1 (AP1)
2025-11-20 | PXD041159 | Pride
Genomics Developmental dynamics of floral gene regulation [ChIP-Seq]
2014-02-03 | E-GEOD-46986 | biostudies-arrayexpress
Genomics Developmental dynamics of DNA accessibility during flower development [DNaseI-Seq]
2014-02-03 | E-GEOD-46894 | biostudies-arrayexpress
Proteomics Galectin-3 Mediated Endocytosis of the Orphan G-Protein-Coupled Receptor GPRC5A
2025-10-09 | PXD048507 | Pride
Proteomics Protein interactions of transcriptional co-regulator LEUNIG_HOMOLOG (LUH) during early stages of Arabidopsis flower development.
2025-11-25 | PXD060520 | Pride
Proteomics Arabidopsis Wdr8 interactors identification
2022-02-17 | PXD026063 | Pride
Proteomics Complementary peptides (cPEPs) modulate protein translation
2023-01-09 | PXD017404 | Pride
Transcriptomics Expression profile pAP1-AP1-GR ap1cal inflorescences - time series (days)
2014-01-01 | E-GEOD-47981 | biostudies-arrayexpress