Dataset Information

Small RNA-seq reveals the effect of formaldehyde exposure on chicken embryo liver microRNA profile

ABSTRACT: Formaldehyde (FA) is commonly used for hatchery disinfection, but its effects on embryonic development remain unclear. MicroRNAs (miRNAs) regulate gene expression post‑transcriptionally and may mediate FA‑induced transcriptional responses. This study investigates the impact of FA exposure on the miRNA landscape in chicken embryo liver. Small RNA‑seq libraries were constructed and sequenced using the Illumina NextSeq platform. Reads were trimmed and quantified. Differential expression analysis was performed with DESeq2. Target genes of differentially expressed miRNAs (DEMs) were predicted (miRDB), and GO/KEGG/Reactome enrichment was conducted. A total of 662 mature miRNAs were detected. Differential expression analysis identified 30 DEMs (11 up‑regulated, 19 down‑regulated). FA exposure at the embryo stage may significantly alter miRNA expression, affecting pathways involved in epigenetic, oxidative stress, and immune regulation. These results warrant reconsidering FA use in poultry hatcheries and further functional validation of miRNA‑target interactions.

ORGANISM(S): Gallus gallus

PROVIDER: GSE295531 | GEO | 2025/12/04

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Captive rearing programs (hatcheries) are often used in conservation and management efforts for at-risk salmonid fish populations. However, hatcheries typically rear juveniles in environments that contrast starkly with natural conditions, which may lead to phenotypic and/or genetic changes that adversely affect the performance of juveniles upon their release to the wild. Environmental enrichment has been proposed as a mechanism to improve the efficacy of population restoration efforts from captive-rearing programs: in this study, we examine the influence of environmental enrichment during embryo and yolk-sac larval rearing at the level of the transcriptome in Atlantic salmon (Salmo salar). Full siblings were reared in either a M-bM-^@M-^\simpleM-bM-^@M-^] environment devoid of structure or a M-bM-^@M-^\complexM-bM-^@M-^] environment enriched with gravel substrate. At the end of endogenous feeding by juveniles, we examined patterns of gene transcription in head tissues using the cGRASP-designed Agilent 4M-CM-^W44K microarray. Significance analysis of microarrays (SAM) indicated that 808 genes were differentially transcribed between rearing environments and a total of 184 gene ontological (GO) terms were over- or under-represented, several of which are associated with mitosis/cell cycle and muscle and heart development. However, there were also pronounced differences among families in gene transcriptional response to rearing environment, with the number of genes significantly differentially transcribed by juveniles in our independent analyses of each family ranging from zero to 3445 (FDR of 5%). Overall, our results suggest that rearing environment enrichment can profoundly change patterns of gene transcription during salmon development, but that the degree of response depends on genetic background. This was a two-condition experiment in which a total of 30 RNA samples isolated from the heads of developing salmon were analysed: 15 juveniles reared in a traditional hatchery environment and 15 reared in a hatchery environment enriched with gravel substrate.

Dataset Information

Small RNA-seq reveals the effect of formaldehyde exposure on chicken embryo liver microRNA profile

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets