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Stage-specific degradation of cyclin D3 orchestrates myelopoiesis and restrains MPN development [RNA-seq]

ABSTRACT: Cyclin D3 is critical for myelopoiesis, with its rapid, stage-specific degradation regulating normal granulocyte-monocyte differentiation. Using a knock-in mouse model with a degradation-resistant cyclin D3 variant (D3T283A), we show that sustained cyclin D3 levels enhance granulocyte-monocyte output, cause microcytic erythrocytes, thrombocytosis, and extramedullary hematopoiesis. Splenic HSPCs from D3T283A mice exhibit increased self-renewal and competitive repopulation advantage. Prolonged D3T283A expression drives a high-penetrance myeloproliferative neoplasm, reducing survival.

ORGANISM(S): Mus musculus

PROVIDER: GSE296131 | GEO | 2026/05/01

REPOSITORIES: GEO

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Stage-specific degradation of cyclin D3 orchestrates myelopoiesis and restrains MPN development [scRNA-seq]

Project description:Cyclin D3 is critical for myelopoiesis, with its rapid, stage-specific degradation regulating normal granulocyte-monocyte differentiation. Using a knock-in mouse model with a degradation-resistant cyclin D3 variant (D3T283A), we show that sustained cyclin D3 levels enhance granulocyte-monocyte output, cause microcytic erythrocytes, thrombocytosis, and extramedullary hematopoiesis. Splenic HSPCs from D3T283A mice exhibit increased self-renewal and competitive repopulation advantage. Prolonged D3T283A expression drives a high-penetrance myeloproliferative neoplasm, reducing survival.

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Stage-specific degradation of cyclin D3 orchestrates myelopoiesis and restrains MPN development [RNA-seq]

Project description:Stage-specific degradation of cyclin D3 orchestrates myelopoiesis and restrains MPN development [RNA-seq]

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Stage-specific degradation of cyclin D3 orchestrates myelopoiesis and restrains MPN development [scRNA-seq]

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