Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

SP5/8 transcription factors drive primary cilia formation

ABSTRACT: Sp5/8 are tissue specific zinc finger transcription factors downstream of Wnt signaling pathway. Sp5/8 null embryos display severe posterior axis truncation resembling mutant phenotypes of Wnt/b-catenin signaling pathway. To address the role of Sp5/8 in gastrulation development, we differentiated R1 Wildtype (WT) and Sp5/8 dko Embryonic Stem Cells (ESCs) as gastruloids as described previously (Beccari, L. et al, 2018) to evaluate scRNA transcriptome analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE296732 | GEO | 2026/04/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Bulk-RNA sequencing of E8.25 posterior tissue +2 somites from controls and Sp5/8 double knockout or Wnt3a knockout embryos

Project description:Sp5/8 are Zn finger downstream transcription factors of Wnt/b-catenin signaling pathway. Deletion of Sp5/8 in mice result in severer posterior axis truncation by E9.5 closely resembling Wnt pathway mutants. The goal of this project is to identify differentially expressed genes between controls and Sp5/8 double knockout mutants that could potentially reveal mechanisms of the role of Sp5/8 factors in self-renewal and differentiation of caudal progenitors.

2025-11-12 | GSE252659 | GEO

The WNT target SP5 negatively regulates WNT transcriptional programs in human pluripotent stem cells

Project description:The WNT/β-catenin signaling pathway is a prominent player in many developmental processes, including gastrulation, anterior-posterior axis specification, organ and tissue development and homeostasis. Here, we use human pluripotent stem cells (hPSCs) to study the dynamics of the transcriptional response to exogenous activation of the WNT pathway. We describe a mechanism involving the WNT target gene SP5 that leads to termination of the transcriptional program initiated by WNT signaling. Integration of gene expression profiles of wildtype and SP5 mutant cells with genome-wide SP5 binding events reveals that SP5 acts to diminish expression of genes previously activated by the WNT pathway. Furthermore, we show that activation of SP5 by WNT signaling is most robust in cells with developmental potential, such as stem cells. These findings indicate a mechanism by which the developmental WNT signaling pathway reins in expression of transcriptional programs.

2017-08-29 | GSE103175 | GEO

Genome-wide binding profiles of Sp5 in differentiation mouse embryonic stem cells

Project description:We have determined the genome-wide binding profile of the transcription factor Sp5. Flag-tagged Sp5 was targeted to the HPRT locus in A2Lox.cre ES cells to allow for Doxycycline inducible expression. ES cells were cultured as embryoid bodies for 2 days to prime them for germ layer differentiation. Cells were then treated with Doxycycline for 24 hrs. We found that Flag-Sp5 prefentially bound to promoters associatd with several growth factor signalling pathways, but most prominently with the Wnt/beta-catenin pathway to selectively promote mesoderm differentiation over neural. These data implicate Sp5 as new regulator of Wnt/beta-catenin target expression to promotes ES cell differentation into the mesoderm lineage. Examination of Sp5 transcription factor binding in differentiating embryonic stem cells.

2016-04-01 | E-GEOD-72989 | biostudies-arrayexpress

Determination of the Sp5 regulated transcriptome in differentiating ES cells

Project description:Sp5 is a target and critical transducer of the Wnt/beta-catenin signaling pathway and is essential for mesoderm differentiation in vertebrate embryos. We established a doxycycline inducible ES cell line to over-express Flag epitope-tagged Sp5 protein in differentiating ES cells which have the capacity to form mesoderm cells in vitro. The goal of this study was to identify the genes/gene networks regulated by Sp5 to understand how it regulates mesoderm differentiation by identifying important target genes which may mediate its activity. Total RNA was extracted from ES cells after 3 days of differentiating following F-Sp5 over-expression from day 2 to 3. We compared the transcriptome of differentially expressed genes between untreated (minus) and doxycycline treated (plus) ES cells to identify Sp5 regulated genes.

2016-04-01 | E-GEOD-73082 | biostudies-arrayexpress

Genome-wide binding profiles of Sp5 in differentiation mouse embryonic stem cells

2016-04-01 | GSE72989 | GEO

Determination of the Sp5 regulated transcriptome in differentiating ES cells

2016-04-01 | GSE73082 | GEO

An evolutionary conserved Sp5-Wnt3 regulatory module contributes to restrict head organizer activity in Hydra

Project description:Head regeneration in Hydra requires the transformation of gastric tissue into a head organizer that produces a head activator and a head inhibitor. Here we report the decipherment of a long-standing question in developmental biology, the identification of the transcription factor Sp5 as a key head inhibitory component. We show that Sp5 has an apical to basal graded expression pattern in intact Hydra, its expression is induced upon Wnt/β-catenin signaling activation and when knocked-down triggers the formation of multiple heads and axes in homeostatic and regenerative conditions. Our data indicate that Sp5 acts in a feed-forward loop to robustly regulate its own expression and that Sp5 prevents head formation by repressing the Wnt3 promoter. This Sp5 mediated Wnt antagonism is conserved and was invented early in metazoan evolution to set up axial patterning.

2019-03-01 | GSE110277 | GEO

Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos

Project description:Although specific transcription factors (TFs) are known to regulate cell fate decisions, the degree to which they can stimulate formation of specific cell organelles is less clear. We used a multiomics comparison of the transcriptomes of ciliated and unciliated embryonic cells to identify TFs up-regulated in ciliated cells. We also used conditional genetics in mouse embryos and stem cells and found that the TFs SP5 and SP8 regulate cilia formation and gene expression. In embryos lacking Sp5 and Sp8, primary and motile cilia were shorter than normal and reduced in number across cell types, contributing to situs inversus and hydrocephalus. Moreover, expression of SP8 was sufficient to induce primary cilia in unciliated cells. This work will facilitate the study of cilia assembly using stem cell models and promote further understanding of human ciliopathies.

2025-06-25 | GSE274844 | GEO

An evolutionarily-conserved Wnt3/β-catenin/Sp5 feedback loop restricts head organizer activity in Hydra [ChIP-Seq]

2019-01-18 | GSE121316 | GEO

Transcription factors SP5 and SP8 drive primary cilia formation in mammalian embryos [ChIP-seq]

2025-06-25 | GSE273920 | GEO