Project description:Analysis of wig-1 pathways via suppression of Wig-1 by antisense oligonucleotides Total RNA obtained from mouse brain subjected to antisense oligonucleotide (ASO) treatement compared to PBS (control), and negative control ASO brain treatment.
Project description:We used C1QTNF1-AS1 antisense oligonucleotide pulldown coupled with MS to identify protein interactors of lncRNA C1QTNF1-AS1 in human HCT116 cells. Five antisense oligonucleotides against C1QTNF1-AS1 and luciferase (negative control) were used for the pulldown. Three independent oligonucleotides (C1QTNF1-AS1 oligonucleotides 1, 3 and 5), efficiently retrieve C1QTNF1-AS1 and extracted proteins from these samples were compared using label-free (LFQ) Quantitative proteomics. C1QTNF1-AS1 oligonucleotides 2 and 4 were excluded from the analysis as they did not retrieve C1QTNF1-AS1.
Project description:We have shown that decreasing psmd9 expression effects lipid metabolism in vivo and were interested in investigating the pathways by which psmd9 elicited these changes. To investigate these mechanisms, we performed proteomic analyses on the livers from C57 and DBA mice that had been treated with antisense oligonucleotides targeted to psmd9.
Project description:We performed high-throughput RNA sequencing to characterize possible differences in the transcriptome of primary human aortic Vascular Smooth Muscle cells abrogated of NEIL3 mRNA via antisense oligonucleotides targeting NEIL3 exon 4, compared with control cells treated with a scramble version of the same antisense oligonucleotide
Project description:Embryos were injected with either control or Smicl antisense morpholino oligonucleotides and harvested at Stage 10.5 for RNA analysis. Keywords: Knock-down analysis
Project description:Embryos were injected with either control or Activin antisense morpholino oligonucleotides and harvested at Stage 10.5 for RNA analysis. Keywords: Knock-down analysis
Project description:To investigate gene expression change induced by modified gapmer antisense oligonucleotides, we performed transcriptome analysis of Scarb1 ASO or PBS treated mouse livers.