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Recruitment, rewiring, and deep conservation in flowering plant gene regulation

ABSTRACT: We conducted single-nuclei transcriptome profiling of multiple tissues from a range of flowering plant species. Individual samples from the same species and tissue were integrated to construct celltype-labeled snRNA atlases for four brassica species and one grass species (available as supplementary files). These atlases were used to track the activity of hundreds of transcription factors (TFs) across species and celltypes, by leveraging genome-wide TF binding site data from accompanying multi-species DAP-seq experiments (DAP-seq data available as SRA BioProject PRJNA1177505). The final integrated dataset revealed clear patterns of regulatory conservation across the plant phylogeny, as well as key examples of regulatory re-wiring to enable new cell types and lineage-specific adaptations.

ORGANISM(S): Camelina sativa Thlaspi arvense Panicum hallii Arabidopsis thaliana Brachypodium distachyon Arabidopsis lyrata Capsella rubella Brassica oleracea Sorghum bicolor

PROVIDER: GSE297576 | GEO | 2025/06/05

REPOSITORIES: GEO

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