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RNA proximity labeling in log phase Caulobacter crescentus NA1000

ABSTRACT: APEX2 RNA proximity labeling is a powerful method for determining localized RNAs in vivo. APEX2 RNA proximity labeling was adapted to bacterial cells, using an APEX2 fusion to the core scaffold of BR-bodies, RNase E. As a control, APEX2 was also fused to a variant of RNase E that lacks its C-terminal IDR and is unable to form BR-bodies, RNase E delta CTD. RNA proximity labeling was performed, and we observed a similar pattern of enriched RNAs to density centrifugation isolated BR-bodies (Al-Husini et al. Mol Cell 2020).

ORGANISM(S): Caulobacter vibrioides NA1000

PROVIDER: GSE297938 | GEO | 2025/05/31

REPOSITORIES: GEO

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