Genomics

Dataset Information

0

An integrated transcription factor framework for Treg identity and diversity


ABSTRACT: Vertebrate cell identity depends on the combined activity of scores of transcription factors (TF). While TFs have often been studied in isolation, a systematic perspective on their integration has been missing. Focusing on FoxP3+ regulatory T cells (Tregs), key guardians of immune tolerance, we combined single-cell chromatin accessibility, machine learning, and high-density genetic variation, to resolve a validated framework of diverse Treg chromatin programs, each shaped by multi-TF inputs. This framework identified previously unrecognized Treg controllers (Smarcc1) and illuminated the mechanism of action of FoxP3, which amplified a pre-existing Treg identity, diversely activating or repressing distinct programs, dependent on different regulatory partners. Treg subpopulations in the colon relied variably on FoxP3, Helios+ Tregs being completely dependent, but RORγ+ Tregs largely independent. These differences were rooted in intrinsic biases decoded by the integrated framework. Moving beyond master regulators, this work unravels how overlapping TF activities coalesce into Treg identity and diversity.

ORGANISM(S): Mus musculus

PROVIDER: GSE298730 | GEO | 2025/06/02

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2015-07-25 | E-GEOD-68009 | biostudies-arrayexpress
2015-07-25 | E-GEOD-71304 | biostudies-arrayexpress
2015-07-25 | GSE68009 | GEO
2015-07-25 | GSE71304 | GEO
2018-05-18 | PXD007744 | Pride
2018-05-18 | PXD005477 | Pride
2018-05-18 | PXD007745 | Pride
2023-12-20 | GSE240657 | GEO
2021-08-03 | GSE164149 | GEO
2021-08-03 | GSE176191 | GEO