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Transcriptomic alterations induced by low-intensity ultrasound (LIUS) in non-small cell lung carcinoma (NSCLC) cell lines.

ABSTRACT: Ultrasound therapy has emerged as a promising, non-invasive modality for cancer treatment, although its underlying mechanisms remain incompletely understood. Immunoblotting analysis revealed that low-intensity ultrasound (LIUS) alters global levels of histone modifications, including monoubiquitinated H2B (H2Bub1), H3K4me3, and H3K27me3, in NSCLC A549 and H1299 cell lines. Given the pivotal role of histone marks in transcriptional regulation, we further investigated the effect of LIUS on transcriptional control in A549 and H1299 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299009 | GEO | 2026/02/25

REPOSITORIES: GEO

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Transcriptomic alterations induced by low-intensity ultrasound (LIUS) in OSCC.

Project description:Ultrasound therapy has emerged as a promising, non-invasive modality for cancer treatment, although its underlying mechanisms remain incompletely understood. Immunoblotting analysis revealed that low-intensity ultrasound (LIUS) alters global levels of histone modifications, including monoubiquitinated H2B (H2Bub1), H3K4me3, and H3K27me3, in oral squamous cell carcinoma (OSCC) OC3 cells. Given the pivotal role of histone marks in transcriptional regulation, we further investigated the effect of LIUS on transcriptional control in OC3 cells.

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BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosis

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Low-Intensity Pulsed Ultrasound Effects on the Human Blood Brain Barrier

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Gene expression profie at single cell level of lung cells in healthy rats and rats with pulmonary hypertension treated with sham-stimulation or ultrasound

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2023-12-01 | GSE247625 | GEO

Identification of early response genes to low-intensity pulsed ultrasound in bone marrow stromal cells

Project description:Low-intensity pulsed ultrasound (LIPUS) has been applied as a therapeutic adjunct to promote fracture healing. However, the detailed molecular mechanisms by which LIPUS promotes bone fracture healing have not yet been fully elucidated. In the present study, the early response genes elicited by low-intensity pulsed ultrasound (LIPUS) in bone marrow stromal cells (BMSCs) were investigated using GeneChip® oligonucleotide microarrays.

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Spatiotemporal local and abscopal cell death and immune responses to histotripsy focused ultrasound tumor ablation

Project description:Histotripsy is a novel focused ultrasound tumor ablation modality with potent immunostimulatory effects. To measure the spatiotemporal kinetics of local and abscopal responses to histotripsy, C57BL/6 mice bearing bilateral flank B16 melanoma or Hepa1-6 hepatocellular carcinoma tumors were treated with unilateral sham or partial histotripsy. Treated and Controltralateral untreated (abscopal) tumors were analyzed using multicolor immunofluorescence, digital spatial profiling, RNA sequencing (RNASeq), and flow cytometry.

2023-01-12 | GSE221448 | GEO

Osteocytes as main responders to Low Intensity Pulsed Ultrasound for the treatment of fracture healing

Project description:Skeletal tissue is known to respond to mechanical stress. Ultrasound stimulation is one of mechanical stress and low intensity pulsed ultrasound (LIPUS) devices have been clinically utilized to promote the fracture healing. However, it is still not clear which skeletal cells, especially osteocytes or osteoblasts, mainly respond to LIPUS stimulation and how they contribute to fracture healing. To examine that, we utilized medaka known to have bones without embedded osteocytes and zebrafish known to have bones with embedded osteocytes as an in vivo model. Interestingly, fracture healing was accelerated by ultrasound stimulation in zebrafish but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocyte, we performed RNA-sequencing with the murine long bone osteocyte Y4 (MLO-Y4) cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation and functional cluster analysis defined that several molecular signatures related in immunity, secretion and transcription. Especially, most of isolated transcription related genes were modulated by LIPUS also in vivo experiments using zebrafish. Target genes analysis showed that inflammatory response and bone formation in fracture healing could be transcriptionally regulated in osteocytes by LIPUS stimulation. Among these transcription genes, early growth response (Egr) 1,2, JunB, Forkhead box Q1 (FoxQ1) and nuclear factor of activated T cells (NFAT) c1 were not altered by LIPUS in medaka unlike zebrafish suggesting that these genes would be key transcriptional regulator of LIPUS-dependent fracture healing through osteocytes. In this study, we revealed bone-equipped osteocytes is necessary for LIPUS-induced promotion of fracture healing through the transcriptional control of target genes presumably to activate neighboring cells involved in fracture healing event.

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2026-03-01 | GSE313669 | GEO

Dynamic Ultrasound Molecular Targeted Imaging of Senescencein Evaluation of Lapatinib Resistance in HER2 Positive Breast Cancer

Project description:Prolonged treatment of HER2+ breast cancer cells with lapatinib (LAP) causes cellular senescence and acquired drug resistance, which often associating with poor prognosis for patients. We aim to explore the correlation between cellular senescence and LAP resistance in HER2+ breast cancer, screening for molecular marker of reversible senescence, and construct targeted nanobubbles for ultrasound molecular imaging and dynamically evaluate LAP resistance. In this study, we established a new cellular model of reversible cellular senescence using LAP and HER2+ breast cancer cells and found that reversible senescence contributed to LAP resistance in HER2+ breast cancer. Then we identified ecto-5'-nucleotidase (NT5E) as a marker of reversible senescence in HER2+ breast cancer. Based on this, we constructed NT5E targeted nanobubbles (NT5E-FITC-NBs) as a new molecular imaging modality which could both target reversible senescent cells and be used for ultrasound imaging. NT5E-FITC-NBs showed excellent physical and imaging characteristics. As an ultrasound contrast agent, NT5E-FITC-NBs could accurately identify reversible senescent cells both in vitro and in vivo. Our data demonstrate that cellular senescence-based ultrasound targeted imaging can identify reversible senescence and evaluate LAP resistance effectively in HER2+ breast cancer, which has the potential to improve cancer outcomes by altering treatment strategies ahead of aggressive recurrences.

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Low-intensity Pulsed Ultrasound regulates osteoclasts mitochondrial function in the treatment of diabetic periodontitis.

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