Transcriptomics

Dataset Information

0

Functional relevance of naturally occurring single nucleotide polymorphisms in bovine leukemia virus-encoded microRNAs.


ABSTRACT: Background: Bovine leukemia virus (BLV) microRNAs (miRNAs) contribute to viral latency and immune evasion. Naturally occurring single-nucleotide polymorphisms (SNPs) within the BLV miRNA cluster have been associated with persistent lymphocytosis in cattle, yet their functional impact remains unclear. Aims: This study aims to (1) evaluate how SNPs in BLV miRNAs affect miRNA biogenesis and mature miRNA levels; (2) determine whether specific SNPs alter miRNA–mRNA interactions and identify affected targets; and (3) characterize transcriptomic changes induced by reference versus SNP-bearing miRNAs. Methods: BLV miRNA loci were PCR-amplified and sequenced from 53 blood samples of infected cattle. Both reference and SNP-containing precursors were cloned into expression vectors and co-transfected with an miRNA-deficient BLV clone into HEK293T cells. Mature miRNA levels were quantified via stem-loop RT-qPCR. Computational target prediction (miRanda) validated changes in mRNA targeting. Gene-expression effects were assessed using Agilent microarrays, with selected findings confirmed by RT-qPCR and subjected to pathway enrichment analysis (IPA). Significance: This work will elucidate how BLV miRNA polymorphisms modify miRNA maturation and target recognition, reshape host gene networks, and contribute to viral persistence and immune modulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299212 | GEO | 2025/06/15

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2017-02-23 | PXD004435 | Pride
2016-03-01 | E-GEOD-73389 | biostudies-arrayexpress
2017-03-30 | MSV000080810 | MassIVE
2020-12-31 | GSE157934 | GEO
2024-08-02 | PXD048367 | Pride
2014-12-15 | GSE40807 | GEO
2013-02-26 | E-GEOD-36121 | biostudies-arrayexpress
2021-07-28 | GSE175466 | GEO
2016-03-01 | GSE73389 | GEO
2017-08-22 | E-MTAB-4874 | biostudies-arrayexpress