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Transcriptomic Profiling Reveals the Impact of ASIC2 Knockdown in Primary Mouse Thalamic Neurons

ABSTRACT: This study investigates the transcriptomic consequences of knocking down the Acid-Sensing Ion Channel Subunit 2 (ASIC2) gene in primary mouse thalamic neurons. Primary thalamic neurons were isolated and cultured at a density of 500,000 cells per well. Three days post-plating, ASIC2 expression was knocked down using specific shRNA constructs. Following the knockdown period, total RNA was extracted from the cells and subjected to bulk mRNA sequencing (RNA-seq) to comprehensively analyze changes in global gene expression profiles. This experimental design aims to identify differentially expressed genes and pathways regulated by ASIC2 in thalamic neurons, providing insights into its functional roles within this specific neuronal population.

ORGANISM(S): Mus musculus

PROVIDER: GSE299302 | GEO | 2025/10/01

REPOSITORIES: GEO

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