Spatial transcriptomic sequencing of fresh frozen canine osteosarcoma tumor samples

ABSTRACT: Osteosarcoma (OSA) is a highly aggressive and heterogeneous bone cancer in dogs and humans, with limited treatment advances over decades. Using the 10x Genomics Visium spatial transcriptomics platform, this study analyzed 14 canine OSA tumors to map gene expression patterns within the tumor microenvironment and correlate them with patient outcomes. Spatial and integrated analyses revealed conserved transcriptional clusters and immune cell infiltration patterns, including macrophage-associated regions in long-term survivors. Deconvolution with single-cell RNA-seq data identified co-localizing immune cell populations such as mast cells with osteoclasts and NKT cells with regulatory dendritic cells. These findings provide insight into tumor heterogeneity and immune landscapes, offering potential targets for future therapies in both species.

ORGANISM(S): Canis lupus familiaris

PROVIDER: GSE299494 | GEO | 2026/04/27

REPOSITORIES: GEO