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ABC1K2 is involved in stress response and glucosinolate modification during seed development in Arabidopsis thaliana

ABSTRACT: The Activity of BC1 complex Kinases (ABC1K) family is a ubiquitous group of proteins that share a highly conserved ABC1 domain (unrelated to the ATP-Binding Cassette – ABC – transporter proteins), whose precise function is still undetermined. The ABC1K proteins are included among the atypical protein kinases due to their lack of many features characterizing canonical eukaryotic protein kinases; despite this, they share a structurally similar kinase fold domain and have been reported to exhibit kinase activity. The Abc1k genes in plants show different functions compared to those in yeast. For instance, in wheat, TaABC1 is involved in a hypersensitive response to fungal pathogens and confers enhanced tolerance to abiotic stresses when overexpressed in Arabidopsis. In rice 15 ABC1K genes were identified, nine of which were localized in chloroplasts and some of them were affected by NaCl, high temperature, methyl viologen, abscisic acid and cadmium. We describe the characterisation of the knock-out mutant lines at the gene ABC1K2, corresponding to the locus At5g24970 in the A. thaliana genome, according to the proposed classification of Lundsquit et al., 2013. The gene seems to be expressed during embryogenesis (as for GUS assay), and ABC1K2 mRNA is not detectable in leaves, shoots or roots. Mutant lines have been analysed, and although the lack of ABC1K2 does not influence the overall plant fitness, development and reproduction, mutant seeds show a reduced ABA content, an increased seed size and mass as well as a reduced seed pigmentation, different accumulation of ABA and glucosinolates.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE299982 | GEO | 2025/12/03

REPOSITORIES: GEO

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