Injury-induced niche factors Cxcl12 and Shh/Ihh coordinate suture stem cell activation during calvarial bone regeneration
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ABSTRACT: Suture stem cells (SuSCs) are important for homeostasis and regeneration of cranial bone and can be used as a model to understand stem cell regulation for bone regeneration at a distance. Using a mouse cranial bone injury model, we identified the chemokine Cxcl12 and the Hedgehog family ligands Shh and Ihh as injury-induced interacting niche factors that coordinately regulate Gli1+ SuSC expansion, directional migration,and osteoblastic differentiation. Cxcl12 increased in the early inflammatory phase after injury, and Cxcl12-mediated activation of its cognate receptor, Cxcr4, in Gli1+ SuSCs promoted bone regeneration by stimulating SuSC proliferation and migration. Cxcl12-Cxcr4 signaling induced Shh and Ihh expression, which promoted Gli1+ SuSC proliferation and osteoblastic differentiation.
ORGANISM(S): Mus musculus
PROVIDER: GSE300214 | GEO | 2025/06/23
REPOSITORIES: GEO
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