Transcriptional study in the brain using control animals from the Eomes-Cre mouse line at embryonic day 16.5 - WT only

ABSTRACT: The specification of neural composition and of neuronal networks in the six-layered cerebral cortex is a highly organized and complex process. Neural stem cells (NSCs) and glutamatergic neurons of the cerebral cortex are born in different stem cell compartments, namely the ventricular (VZ) and the subventricular zone (SVZ). Embryonic VZ and SVZ-derived progenitors produce neurons of the upper layers (UL) and deep layers (DL) of the stratified neuroepithelium in an inside-out manner. Both cellular compartments seem to be associated with higher cognitive tasks that are observed in these species. Understanding of the molecular mechanisms that control cell fate within the SVZ is therefore of utmost interest to understand one basis of cognition. Furthermore, the SVZ is one of the stem cell niches of the adult nervous system and thus contributes to repair processes after CNS injuries in mice. Recent data from embryonic and neural stem cells (ESC, NSC) revealed that chromatin modifying enzymes (CME) and epigenetic modifications play a pivotal role in cell fate decisions such as proliferation/self-renewal and lineage differentiation. In addition, during development the balance between proliferation and differentiation is achieved through the activities of diverse transcription factors (TF) that are expressed in a time- and space-specific manner. The aim of this project is to further elucidate the role of neural stem cells that are residing in the SVZ with a focus on the implications of epigenetic modifications mediated by MLLT3 in transcriptional control and regulation of proliferation, survival and differentiation during brain development.

ORGANISM(S): Mus musculus

PROVIDER: GSE300653 | GEO | 2025/07/30

REPOSITORIES: GEO