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Clariom D Pico gene array of primary GBM cells grown as 3D (LD⁺) spheroid cultures or 2D (LD⁻) cultures

ABSTRACT: In this study, we aimed to elucidate the molecular underpinnings of metabolic adaptation in the stressed TME. LD accumulation has been well documented in glioblastoma (GBM), a prototypical high-grade brain malignancy characterized by severe acidosis, hypoxia, and metabolic heterogeneity. Unexpectedly, we observed a prominent enrichment of pathways related to glycocalyx remodeling in the LD-rich niche of patient tumors, particularly those involving CS biosynthesis and PG regulation. These findings prompted us to explore how glycan remodeling intersects with lipid metabolism in GBM and whether this interaction contributes to tumor stress adaptation. Our results highlight an acidosis-induced glycan program with potential as a metabolic vulnerability, offering new therapeutic avenues for targeting the lipid-stressed niche in aggressive tumors. We used microarrays to evaluate transcriptional signature realted to 3D (LD⁺) cultures compared to 2D (LD⁻) cultures from primary glioblastoma (GBM) cell line.

ORGANISM(S): Homo sapiens

PROVIDER: GSE300771 | GEO | 2026/01/04

REPOSITORIES: GEO

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