Spatial transcriptome of pre-invasive lung adenocarcinoma

ABSTRACT: We observed distinct mutational patterns across stages: EGFR mutations increased with pathological stages, while non-V600E BRAF mutations were enriched in AAH and AIS, suggesting pre-invasive lesions harboring different drivers follow distinct evolutionary paths. Based on these differences, we derived 23-gene and 59-gene signatures that stratified pre-invasive lesions into aggressive and non-aggressive groups. The aggressive signature was associated with worse prognosis supported by five stage-I LUAD cohorts. Two aggressive signature genes, LPGAT1 and XPR1, were functionally validated to promote LUAD cell invasion. Compared to non-aggressive lesions, aggressive lesions exhibited higher tumor mutation burden, enrichment of proliferative and EMT-related pathways, and evidence of immune-stromal remodeling.

ORGANISM(S): Homo sapiens

PROVIDER: GSE300827 | GEO | 2026/06/30

REPOSITORIES: GEO