Project description:Doxorubicin is considered one of the most potent established chemotherapeutics in the treatment of liposarcoma; however, the response rates usually below 30%, are still disappointing. This study was performed to identify gene expression changes in liposarcoma after doxorubicin treatment. Cells of 19 primary human liposarcoma were harvested intraoperatively and brought into cell culture. Cells were incubated with doxorubicin for 24 h, RNA was isolated and differential gene expression was analysed by the microarray technique. We used microarrays to detail the global gene expression changes following doxorubicin treatment of primary liposarcoma cell cultures Experiment Overall Design: We compared untreated primary cell cultures obtained from liposarcoma treated with doxorubicin treated cultures to determine global gene expression changes by microarray analysis
Project description:Doxorubicin is considered one of the most potent established chemotherapeutics in the treatment of liposarcoma; however, the response rates usually below 30%, are still disappointing. This study was performed to identify gene expression changes in liposarcoma after doxorubicin treatment. Cells of 19 primary human liposarcoma were harvested intraoperatively and brought into cell culture. Cells were incubated with doxorubicin for 24 h, RNA was isolated and differential gene expression was analysed by the microarray technique. We used microarrays to detail the global gene expression changes following doxorubicin treatment of primary liposarcoma cell cultures Keywords: response to chemotherapeutic agent
Project description:Primary mediastinal liposarcoma (MLPS) is an extremely rare tumor with unclear molecular biology. We conducted RNA sequencing in five cases of MLPS and compared gene expression between dedifferentiated liposarcoma (DDLPS) and well-differentiated liposarcoma (WDLPS). Enrichment analyses and immune cell deconvolution revealed that DDLPS showed features of dedifferentiation, cell cycle activation, Wnt signaling, and immune-cold status. This dataset provides the first transcriptomic landscape of primary MLPS and offers insight into potential therapeutic targets.
Project description:In order to identify new key molecules in the pathogenesis of myxoid liposarcoma, we performed comparative gene expression profiling in myxoid liposarcoma and fat tissue samples.