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Transcriptomic analysis of gene expression pathways influenced by toxic chemical exposure on the Caenorhabditis elegans cuticle.

ABSTRACT: Here, we propose that the cuticle of the nematode Caenorhabditis elegans (C. elegans) can be used as a model for human skin to test potentially toxic compounds, in particular, those commonly used in skincare and household products. We examined the effect of toxic chemicals on cuticle morphology and mechanical behavior using atomic force microscopy (AFM) and our findings revealed that two parameters, surface roughness and elastic modulus, are good markers for quantifying cuticle damage caused by chemicals. The damage caused by the chemicals correlated to their toxicity, as expressed in terms of maximum concentration allowed by EU regulations for use in skincare and household products. Transcriptomic analysis showed us that each chemical elicited a specific response in terms of differential gene expression, suggesting the lack of a general response to chemical exposure from the worms in this study. It also showed that a large number of genes involved in the synthesis of cuticle proteins, as well as in metabolism and enzyme activity, were affected by chemical exposure. The number of genes with altered expression levels correlated with chemical toxicity, as well as with level of morphological and mechanical changes in the cuticle. The analysis of affected genes provides a pathway to understand the molecular mechanisms of toxic chemical action. Overall, we show that the cuticle of C. elegans is a good model for human skin for toxicological screening, and this approach will be of particular interest to the cosmetics and household chemical industries.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE300950 | GEO | 2025/07/01

REPOSITORIES: GEO

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Transcriptomic analysis of gene expression pathways influenced by toxic chemical exposure on the Caenorhabditis elegans cuticle.

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