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Impact of SliP4 deletion on the high-light acclimation in Synechocystis sp. PCC 6803

ABSTRACT: SliP4 is a small, 37 amino acids protein that is strongly induced when the cyanobacterium Synechocystis sp. PCC 6803 is exposed to high-light conditions. Deletion mutants of the sliP4 gene manifest a light-sensitive phenotype due to impaired cyclic electron flow and state transitions. In this study, we aimed to investigate the consequences of SliP4 deficiency on the process of high-light acclimation. The sliP4 deletion mutant demonstrated a complete gene regulatory response 30 minutes after the light intensity was increased from 50 to 250 μmol photons m-2 s-1, a process that was mediated by the RpaB-PsrR1 system. However, after the shift to high light, the mutant increased the production of extracellular polysaccharides and the cells began to aggregate, thereby effectively reducing the potential impact of light stress effects caused by the impaired capacity for cyclic electron flow and state transitions. This effect included the upregulation of xssA-E and xssN-P genes for the production of the sulfated exopolysaccharide synechan. Our results demonstrate that the unicellular cyanobacterium Synechocystis attempts to compensate for the crucial role of SliP4 by activating a population-level response to cope with light stress conditions, thereby revealing several genes involved in this response.

ORGANISM(S): Synechocystis sp. PCC 6803

PROVIDER: GSE302282 | GEO | 2026/03/11

REPOSITORIES: GEO

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Impact of SliP4 deletion on the high-light acclimation in Synechocystis sp. PCC 6803

Project description:SliP4 is a 37 amino acids small protein that is strongly induced when the cyanobacterium Synechocystis is exposed to high-light conditions. Deletion mutants exhibit a light-sensitive phenotype due to impaired cyclic electron flow and state transitions. Here, we investigated the consequences of SliP4 deficiency on the high-light acclimation. The ∆sliP4 mutant exhibited a fully intact gene regulatory response 30 min after shifting the light intensity from 50 to 250 μmol photons m-2 s-1 mediated by the RpaB-PsrR1 system. However, 48 h after the shift to high light, the mutant increased the production of extracellular polysaccharides and the cells started to aggregate, effectively reducing the potential impact of light stress effects caused by the impaired capacity for cyclic electron flow and state transitions. This effect included the upregulation of xssA-E and xssN-P genes for the production of synechan, a sulfated exopolysaccharide. Our results show that the unicellular cyanobacterium Synechocystis can compensate the crucial role of SliP4 by the activation of a population-level response to cope with light stress conditions and reveal several genes involved in this response.

2026-02-09 | PXD065318 | Pride

The gene sml0013 of Synechocystis sp. strain PCC 6803 encodes for a novel subunit of the NDH1 complex that is ubiquitous distributed among cyanobacteria.

Project description:The NDH1 complex fulfils numerous tasks in the cyanobacterial cell such as respiration, cyclic electron flow, and inorganic carbon concentration. Despite the immense progress in our understanding of structure/function relation of the cyanobacterial NDH1 complex, the subunits catalysing the NAD(P)H docking and oxidation are still missing. The gene sml0013 of Synechocystis 6803 encodes for a small protein of unknown function for that homologs exist in all completely known cyanobacterial genomes. The protein exhibits weak similarities to the NDF6 protein, which was reported from Arabidopsis chloroplasts as a NDH subunit (Ishikawa et al. 2008). A sml0013 inactivation mutant of Synechocystis 6803 was generated and characterized. It showed only weak differences regarding growth and pigmentation at various culture conditions; most remarkably it exhibited a glucose-sensitive phenotype in the light. The genome-wide expression pattern of the M-NM-^Tsml0013::Km mutant was almost identical to wild type when grown under high CO2 conditions as well as after shifts to low CO2 conditions. However, measurements of the photosystem I redox kinetic in cells of the M-NM-^Tsml0013::Km mutant revealed differences to wild type such as a decreased capability of cyclic electron flow as well as of utilization of electrons from catabolic processes. These results suggest that the Sml0013 protein (named NdhP) represent a novel subunit of the cyanobacterial NDH1 complex mediating its coupling to the respiratory or photosynthetic electron flow. Gene expression of Synechocystis sp. PCC 6803 WT and a M-NM-^Tsml0013::Km mutant was monitored at HC conditions (5% CO2) and at 24h after a shift to LC conditions (ambient air containing 0.035% CO2). Each condition was sampled in biological duplicates.

2013-06-29 | E-GEOD-48415 | biostudies-arrayexpress

The gene sml0013 of Synechocystis sp. strain PCC 6803 encodes for a novel subunit of the NDH1 complex that is ubiquitous distributed among cyanobacteria.

Project description:The NDH1 complex fulfils numerous tasks in the cyanobacterial cell such as respiration, cyclic electron flow, and inorganic carbon concentration. Despite the immense progress in our understanding of structure/function relation of the cyanobacterial NDH1 complex, the subunits catalysing the NAD(P)H docking and oxidation are still missing. The gene sml0013 of Synechocystis 6803 encodes for a small protein of unknown function for that homologs exist in all completely known cyanobacterial genomes. The protein exhibits weak similarities to the NDF6 protein, which was reported from Arabidopsis chloroplasts as a NDH subunit (Ishikawa et al. 2008). A sml0013 inactivation mutant of Synechocystis 6803 was generated and characterized. It showed only weak differences regarding growth and pigmentation at various culture conditions; most remarkably it exhibited a glucose-sensitive phenotype in the light. The genome-wide expression pattern of the Δsml0013::Km mutant was almost identical to wild type when grown under high CO2 conditions as well as after shifts to low CO2 conditions. However, measurements of the photosystem I redox kinetic in cells of the Δsml0013::Km mutant revealed differences to wild type such as a decreased capability of cyclic electron flow as well as of utilization of electrons from catabolic processes. These results suggest that the Sml0013 protein (named NdhP) represent a novel subunit of the cyanobacterial NDH1 complex mediating its coupling to the respiratory or photosynthetic electron flow.

2013-06-29 | GSE48415 | GEO

Resequencing of a mutant bearing an iron starvation recovery phenotype defines Slr1658 as a new player in the regulatory network of a model cyanobacterium

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2017-08-22 | GSE102876 | GEO

The cyanobacterial phytochrome 2 regulates the expression of motility-related genes through the second messenger cyclic di-GMP

Project description:The cyanobacterial phytochrome Cph2 is a light-dependent diguanylate cyclase producing the second messenger c-di-GMP. Under blue light, the Cph2-dependent increase in the cellular c-di-GMP concentration leads to inhibition of motility in the cyanobacterium Synechocystis 6803. However, the targets of c-di-GMP in this cyanobacterium and its mechanism of action remained unclear. Here, we determined the cellular concentrations of three cyclic nucleotides in wild-type and Δcph2 cells after blue- and green light illumination. Inactivation of the photoreceptor gene completely abolished the blue-light dependent increase in the c-di-GMP content. Microarray analysis revealed that in the wild type in comparison to the Δcph2 mutant, blue light mainly led to a change in accumulation of mRNAs encoding minor pilins, putative chaperone usher pili as well as several chemotaxis regulators. The mRNA encoding the minor pilins pilA5-pilA6 is negatively affected by high c-di GMP content under blue light, whereas the minor pilin encoding operon pilA9-slr2018 accumulates under the same conditions, suggesting opposing functions of the respective gene sets. Based on mutational and gene expression analysis, we further suggest that the second Synechocystis 6803 homolog of a CRP-like transcription factor, SyCRP2, is the regulator of minor pilin gene expression and of putative chaperone usher pili genes slr1667/slr1668. Thus, our work indicates that the Cph2-mediated increase in cellular c-di-GMP concentration upon blue-light illumination specifically changes the transcriptome of Synechocystis 6803.

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2015-03-30 | E-GEOD-59371 | biostudies-arrayexpress

The small, high-light-induced protein SliP4 is involved in the proper organization of photosynthetic complexes in thylakoid membranes of Synechocystis sp. PCC 6803

Project description:An increasing number of small proteins has been identified in the genomes of well-annotated model organisms including photosynthetic cyanobacteria such as Synechocystis sp. PCC 6803. A newly assigned protein comprising 37 amino acids was analyzed that is encoded upstream of the super-oxide dismutase SodB encoding gene. The initial hypothesis that this small protein might be functionally related to SodB could not be supported, instead we provide evidence that it fulfills important functions related to the organization of photosynthetic complexes. Therefore, we named it small light-induced protein of 4 kDa, SliP4. SliP4 is strongly induced under high-light-conditions and it is co-localized to photosystem I and II as well as NDH1 complexes on thylakoid membranes. Tagged SliP4 was used to purify NDH1 complexes, where it is especially associated with the subunits NdhF1 and NdhD1. Mutation of the protein-coding part in the sliP4 gene resulted in a high-light-sensitive phenotype, because the mutant was not further able to induce cyclic electron flow and state transitions under high-light conditions. Collectively our data indicate that SliP4 contributes to the association of NDH1 with the photosystem I and/or photosystem II complexes to facilitate different electron transfer modes under high light conditions.

2023-06-07 | PXD036788 | Pride

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Project description:Gene expression changes were monitored in cultures of the cyanobacterium Synechocystis sp. PCC 6803 at 30 min after a shift from cultivation under standard light conditions (50 µmol photons m-2 s-1) to high light (HL; 250 µmol photons m-2 s-1) and 1 h after a shift from the standard growth temperature of 30°C to 20°C (cold). Acclimation to shifts in light and temperature is critical for photoautotrophic organisms such as cyanobacteria. The RRM domain proteins Rbp2 and Rpb3 are RNA-binding proteins that bind specific mRNAs and are involved in the localization of these mRNAs to the thylakoid membrane. Here, we investigated the effects of Rpb2 and Rbp3 on gene expression changes during the acclimation responses to an increase in light intensity and a decrease in temperature. We analyzed cultures of the wild-type (WT) and of the Δrbp2Δrbp3 double mutant, which lacks Rbp2 and Rbp3. We found that the transcript levels of psaA and psaB genes were significantly decreased in cells of the Δrbp2Δrbp3 double mutant upon cold stress and 30 min of high light compared to wild type. In addition, the transcript levels of Rbp1, another RRM domain-containing protein, were greatly increased.

2025-06-10 | GSE269515 | GEO

Synechocystis sp. proteome on differnt light conditions

Project description:Cyanobacteria are an integral part of the Earth’s biogeochemical cycles and a promising resource for the synthesis of renewable bioproducts from atmospheric CO2. Sustainable industrial applications, however, are still scarce and the true limits of phototrophic production remain unknown. One of the limitations of further progress is our insufficient understanding of the quantitative changes in photoautotrophic metabolism that occur during growth in dynamic environments. Uncovering principles of allocation of the key metabolites or proteins as a response to changing environment can provide significant advances in our understanding of phototrophic production limits. However, resolving such complex question requires multidisciplinary approach combining highly-controlled cyanobacteria cultivation coupled to quantitative analyses of specific cellular components. The following datase represents proteomic analysis of the model cyanobacterium Synechocystis sp. PCC 6803 that was cultivated in the well controlled environment of laboratory-scale photobioreactors in the quasi-continuous regime that allowed for maintaining the cells in the exponential growth phase. The sampels were taken under light-limited (25 - 220 µmol(photons) m-2 s-1), optimal (440 µmol(photons) m-2 s-1) and photoinhibited growth (1100 µmol(photons) m-2 s-1). Each culture was sampled in five biological replicates.

2019-01-07 | PXD009626 | Pride

Adaptive evolution provides a high-light stress tolerance in Synechocystis sp. PCC 6803

Project description:we obtained a HL tolerant (Tol) strain Synechocystis sp. PCC 6803 by adaptive evolution experiment that the cells were repeatedly subcultured for prolonged periods of time (52 days) under high light stress condition (7000 to 9000 μmol m-2 s-1). Although the growth of the parental strain almost stopped under 9000 μmol m-2 s-1, no growth inhibition was observed in the Tol strain. Furthermore, the growth rate was identical to that of parental strain under low light condition (40 μmol m-2 s-1). To further investigate the high light tolerant mechanisms in the Tol strain, the transcriptome was performed. The transcriptome data suggests the increase of isiA expression in the Tol strain under HL condition. The overexpression of isiA successfully enhanced the HL stress tolerance in the parental strain. The HL tolerant mechanism was different from previous reported mechanisms, such as a reduction of the light-harvesting antenna size. The tolerant strain would be an attractive host for bio-production under high light conditions.

2020-05-06 | GSE149892 | GEO

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