Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Generation of expandable hepatocyte organoids with multipolar organization from human embryonic stem cells

ABSTRACT: In this study, we successfully generated mature grape-like heporgs (G-heporg) from human embryonic stem cells (hESCs) using a modified hepatocyte organoid culture system. Within this differentiation platform, spherical hepatocyte organoids (S-heporg) spontaneously emerged alongside G-heporgs. Similar to primary heporgs, differentiated G-heporgs exhibited high ALB expression and expansion capability, whereas the S-heporgs lacked both characteristics. Comparative analysis revealed that IGF2 stimulates G-heporg formation. Moreover, we found that targeted modulation of the Hippo-YAP pathway overcame long-term expansion limitations in G-heporgs. Crucially, the differentiated and expandable G-heporgs developed multipolar architectures with functional BC networks, recapitulating the polarity characteristic of primary hepatocytes. Finally, we modeled copper ion metabolism using G-heporgs, demonstrating their utility for investigating polarized trafficking processes of hepatocyte metabolites

ORGANISM(S): Homo sapiens

PROVIDER: GSE302458 | GEO | 2026/04/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Generation of expandable hepatocyte organoids with multipolar organization from human embryonic stem cells

Project description:Generation of expandable hepatocyte organoids with multipolar organization from human embryonic stem cells

| PRJNA1291027 | ENA

Generation of Human Expandable Limb Bud Cells via Chemically Induced Dedifferentiation Strategy [bulk RNA-seq]

Project description:Here, we introduce a novel chemically induced dedifferentiation approach that reverses the cellular development from a differentiated state to progenitor state, conferring the generation of limb bud progenitors from human adult mesenchymal stromal cells and dermal fibroblasts. These chemically induced limb bud cells (hCiLB cells) show a high degree of transcriptomic similarity to human embryonic limb bud cells. We found that hCiLB cells can undergo extensive expansion, while maintaining population homogeneity and long-term self-renewal capacity. Moreover, hCiLB cells exhibit increased osteochondrogenic differentiation ability than their parental cells, providing a new platform for the development of authentic articular cartilage. These results also highlight a potential therapeutic approach for the repair of damaged human tissues through reversal of developmental pathways from mature cells to expandable progenitor cells.

2024-09-01 | GSE243930 | GEO

Generation of Human Expandable Limb Bud Cells via Chemically Induced Dedifferentiation Strategy [scRNA-seq]

Project description:Here, we introduce a novel chemically induced dedifferentiation approach that reverses the cellular development from a differentiated state to progenitor state with reduced senescence features, conferring the generation of limb bud progenitors from human adult mesenchymal stromal cells and dermal fibroblasts. These chemically induced limb bud cells (hCiLB cells) show a high degree of transcriptomic similarity to human embryonic limb bud cells. We found that hCiLB cells can undergo extensive expansion, while maintaining population homogeneity and long-term self-renewal capacity. Moreover, hCiLB cells exhibit increased osteochondrogenic differentiation ability than their parental cells, providing a new platform for the development of authentic articular cartilage. These results also highlight a potential therapeutic approach for the repair of damaged human tissues through reversal of developmental pathways from mature cells to expandable progenitor cells.

2024-09-01 | GSE243929 | GEO

Establishment of stably expandable induced myogenic stem cells by defined factors

Project description:Numerous studies have been conducted to improve the supply of myogenic stem cells for patients with muscle disease, including Duchenne muscular dystrophy. Collecting muscle samples from patients in order to obtain myoblasts or satellite cells is very invasive. Thus, a new method for obtaining myogenic stem cells is required. Here, we established stably expandable induced myogenic stem cells (iMSCs) with defined factors. The iMSCs showed high expression levels of Pax7, Myf5, and MyoD. Also, the iMSCs differentiate into myotubes which are multinucleated fiber. Additionally, the iMSCs differentiated into myotubes, which are multinucleated fibers. We confirmed its myogenic differentiation capacity in mdx mice by detecting dystrophin-positive cells in iMSCs-injected TA muscles. The iMSCs showed higher proliferation capacity than MDSCs in both in vitro and vivo. This study suggests the possibility to apply directly converted expandable myogenic stem cells to muscle disease patients.

2018-11-06 | GSE94506 | GEO

Flare Type Self-expandable Metal Stent for Malignant Colorectal Obstruction

Project description:This study compares flare type self-expandable metal stent with conventional D-type self-expandable metal stent for malignant colorectal obstruction.

| 2278863 | ecrin-mdr-crc

Gene expression analyses of expandable hepatocytes in culture

Project description:To compare the global expression profiles among primary hepatocytes and expandable hepatocytes with or without in vitro differentiation.

2015-11-18 | GSE75141 | GEO

RNA-seq analysis of human expandable arterial endothelial precursors (eAEPs), expandable mesenchymal precursors (eMPs), and controls

Project description:RNA-seq analysis of human expandable arterial endothelial precursors (eAEPs), expandable mesenchymal precursors (eMPs), and controls

| PRJNA418736 | ENA

RNA-seq analysis of grape-like organoids and others in iPSC derived Hepatocyte organoids (iHOs)

Project description:Under iHO conditions, distinctive grape-like organoids emerge. To evaluate the hepatocyte characteristics of grape-like organoids and other organoid forms, grape-like organoids were isolated under a microscope, and RNA-seq was performed.

2025-05-13 | GSE285436 | GEO

Gene expression profile of non-hepatocyte cells of the liver of wild-type and Alb/MDA-9 mice

Project description:We created a mouse line with hepatocyte-specific overexpression of the oncogene MDA-9/SDCBP (Alb/MDA-9). We wanted to check how overexpression of MDA-9 in hepatocytes affect gene expression in surrounding non-parenchymal cells. We isolated non-hepatocyte cells from WT and Alb/MDA-9 mice and performed scRNA-seq in these samples.

2023-06-14 | GSE206096 | GEO

Transcriptome comparisons of "expandable hemangioblasts" (eHBs) and their progeny to control cells

Project description:We examined the transcriptomes of murine "expandable hemangioblasts" (eHBs) and their blood and endothelial progeny, comparing them to the transcriptomes of murine embryonic stem (ES) cells, primary murine endothelial cells isolated from E11.5 yolk sacs or embryos, and E14.5 fetal liver hematopoietic stem cells. Total RNAs were purified from lysates of cultured or primary cells, reverse transcribed, and sequenced on an Illumina HiSeq 2500.

2014-11-13 | E-GEOD-60896 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data