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Integrative Clonal Tracing and CRISPR Screening Identify Temporal and Microenvironmental Determinants in Ovarian Cancer Metastasis

ABSTRACT: We used scRNA-seq (10x Genomics) coupled with CITE-seq based HTO labeling to profile and compare transcriptomes of omentums and ascital fluid harvested from naive or metastasis bearing wild type C57Bl/6 mice. There are three samples from different animals (M-HTOs) that were pooled for loading onto one 10x Chromium lane + eight samples/M-HTOs pooled for a second lane. Later, three seperate libraries were prepared from each 10x lane: HTO, cell barcode (LARRY), and transcriptome. Within the cell barcode libraries: BC.ID1, BC.ID2, BC.ID3, BC.ID4, BC.ID5 and BC.ID6 are used to distinguish different timepoints of injection - early, mid, and late seeding in descending order (meaning BC.ID6 was earliest timepoint, BC.ID1 is latest timepoint).

ORGANISM(S): Mus musculus

PROVIDER: GSE302619 | GEO | 2025/08/27

REPOSITORIES: GEO

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