IPSc-to-thymic epithelial progenitor differentiation under multifactorial DoE optimization and transcriptomic profiling

ABSTRACT: This study investigates the differentiation of human induced pluripotent stem cells (iPSc) into thymic epithelial progenitors (TEPs) through a combinatorial experimental design (Design of Experiments, DoE). Bulk RNA-seq was performed across 96 differentiation conditions covering key developmental transitions: definitive endoderm (DE), anterior foregut endoderm (AFE), third pharyngeal pouch endoderm (3PPE), and thymic epithelial progenitors (TEP). The multifactorial screen leveraged Plackett-Burman designs and transcriptomic similarity to in vivo pharyngeal development atlases to identify optimal differentiation cues.

ORGANISM(S): Homo sapiens

PROVIDER: GSE302942 | GEO | 2025/11/21

REPOSITORIES: GEO