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Cannabidiol exerts anti-inflammatory effects but maintains T effector memory cell differentiation: A Single-Cell Study in Humans [scRNA-seq]

ABSTRACT: Cannabidiol is widely available and often used for pain management. Individuals with kidney disease or renal allografts have limited analgesia options. We conducted a Phase 1 healthy volunteer study to compare the peripheral immune cell distribution before (pre-cannabidiol) and after exposure to cannabidiol at steady state (post-cannabidiol). This study included specimens from 12 participants who received oral cannabidiol (up to 5 mg/kg twice daily) for 12 days. Lymphocytes were isolated and stimulated with anti-CD3/CD28 antibodies, with or without tacrolimus. Understanding the clinical safety of cannabidiol use is important given the paucity of pain control options available for immunocompromised transplant populations. Each individual has three conditions that are sequenced for the phase No CD3/CD28, CD3/CD28 and CD3/CD28 +Tacrolimus across two phases: pre-CBD and post-CBD.

ORGANISM(S): Homo sapiens

PROVIDER: GSE303537 | GEO | 2025/11/07

REPOSITORIES: GEO

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Cannabidiol exerts anti-inflammatory effects but maintains T effector memory cell differentiation: A Single-Cell Study in Humans [snRNA-Seq]

Project description:Cannabidiol is widely available and often used for pain management. Individuals with kidney disease or renal allografts have limited analgesia options. We conducted a Phase 1 healthy volunteer study to compare the peripheral immune cell distribution before (pre-cannabidiol) and after exposure to cannabidiol at steady state (post-cannabidiol). This study included specimens from participants who received oral cannabidiol (up to 5 mg/kg twice daily) for 12 days. Lymphocytes were isolated and stimulated with anti-CD3/CD28 antibodies, with or without tacrolimus. Understanding the clinical safety of cannabidiol use is important given the paucity of pain control options available for immunocompromised transplant populations. Each individual has three conditions that are sequenced for the phase No CD3/CD28, CD3/CD28 and CD3/CD28 +Tacrolimus across two phases: pre-CBD and post-CBD.

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Effects of (-)-cannabidiol on human sebocytes

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Based on multi-omics analysis, the mechanism of cannabidiol improving SNL mouse model of pain with depression was analyzed

Project description:Neuropathic pain (NP) has a high incidence in the general population, is closely related to anxiety disorders, and has a negative impact on the quality of life. As a natural product, Cannabidiol (CBD) has been widely studied for its potential therapeutic effects on symptoms such as pain and depression (DP). However, the mechanism by which CBD improves neuropathic pain with depression is not fully understood. Therefore, we used multi-omics studies (transcriptomics combined with proteomics) to deeply explore the intersection genes of CBD treatment of NP and DP. Secondly, the core targets were screened by protein-protein interaction (PPI) network, gene ontology (GO), Kyoto Encyclopedia of genes and Genomes (KEGG) analysis, molecular docking and molecular dynamics simulation. Behavioral tests were performed to evaluate the effects of CBD intervention on pain and depression behavior in mice with spinal nerve ligation (SNL), and a dose-response curve was drawn. After determining the optimal intervention dose, the core targets and signaling pathways were verified by Western blotting (WB) and quantitative polymerase chain reaction (qPCR). Finally, we investigated the underlying mechanism of CBD by Nissl staining, immunofluorescence (IF) and transmission electron microscopy (TEM). This study aims to provide further theoretical and experimental basis for the discovery of CBD as a potential therapeutic agent.

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Effects of (-)-cannabidiol on human sebocytes

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Comparative Transcriptomics of Broad-Spectrum and Synthetic Cannabidiol Treated Skeletal Myotubes

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