Project description:Chromatin immunoprecipitation from tammar wallaby pouch young cells for DNA bound to CENP-A Chromatin from one Tammar wallaby cell line, total of two technical replicates, 1/8th plate each, IgG and No Antibody IPs performed but not sequenced
Project description:Small RNA isolation of miRNAs, crasiRNAs and piRNAs for RNAseq from tammar wallaby pouch young RNA-seq of various small RNA classes in tammar wallaby
Project description:In this study we use microarray technology to identify differentially expressed genes in the tammar wallaby mammary gland during the lactation cycle. We have focused on gene expression changes in the tammar mammary gland as it shifts from one lactation phase to the next.
Project description:High throughput sequencing of miRNAs collected from tammar milk at different time points of lactation showed high levels of miRNA secreted in milk and allowed the identification of differentially expressed milk miRNAs during the lactation cycle as putative markers of mammary gland activity and functional candidate signals to assist growth and timed development of the young. Comparative analysis of miRNA distribution in milk and blood serum suggests that milk miRNAs are primarily expressed from mammary gland rather than transferred from maternal circulating blood, likely through a new putative exosomal secretory pathway.
Project description:High throughput sequencing of miRNAs collected from tammar milk at different time points of lactation showed high levels of miRNA secreted in milk and allowed the identification of differentially expressed milk miRNAs during the lactation cycle as putative markers of mammary gland activity and functional candidate signals to assist growth and timed development of the young. Comparative analysis of miRNA distribution in milk and blood serum suggests that milk miRNAs are primarily expressed from mammary gland rather than transferred from maternal circulating blood, likely through a new putative exosomal secretory pathway. 8 profiles were produced. Duplicates of day175
Project description:The tammar blastocyst remains in embryonic diapause until it receives a signal from the uterine endometrium via its secretions.This study analaysed uterine flushings from diapause, different stages of reactivation upto late gestation.
Project description:We find that CENP-T acts as a bridge between two well-positioned CENP-A nucleosomes that are present on young alpha-satellite dimers that dominate functional human centromeres. CENP-T is centered over the CENP-B box, where it interacts with the CENP-B/CENP-C complex. Upon cross-linking, the entire CENP-A/CENP-C/CENP-T-containing complex is recovered as a nuclease-protected particle over an alpha-satellite dimer that comprises the fundamental unit of kinetochore chromatin. Our work reveals that CENP-A/CENP-C and CENP-T branches of kinetochore assembly are physically integrated.