Human DCM-time machine unravels cell state changes during primitive gut tube differentiation [RNA-seq]

ABSTRACT: Cell state changes in development and differentiation are directed by gene and enhancer activity dynamics, which are difficult to study in real time. We introduce the DCM-time machine (DCM-TM) into human induced pluripotent stem cells (iPSCs) to enable retrospective, genome-wide tracing of transcriptional activity. This system labels active genes and enhancers with DCM methylation marks that are propagated during S-phase. Applied to iPSC differentiation towards definitive endoderm and primitive gut tube, DCM-TM resolves activity dynamics and enables integration with transcription factor networks and CpG methylation profiles. Together, DCM-TM provides a powerful platform for dissecting both healthy and pathogenic embryogenesis.

ORGANISM(S): Homo sapiens

PROVIDER: GSE304232 | GEO | 2026/09/11

REPOSITORIES: GEO