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Inhibition of Retinoic Acid Receptor Gamma Improves Bovine Embryo Development

ABSTRACT: Retinoic acid receptor gamma (RARγ) plays a critical but poorly understood role in early embryo development and stem cell pluripotency. Here, we investigated the effects of the RARγ-specific inhibitor LY2955303 (LY) on in vitro-produced bovine embryos. Treatment with LY significantly increased blastocyst rates and quality, demonstrating its potential to enhance IVF outcomes in cattle. Single-embryo RNA sequencing using Smart-seq indicated that LY promotes metabolic reprogramming (upregulating glycolysis and TCA cycle activity) while suppressing apoptosis and inflammatory responses. LY- treatment starting from 16-cell stage led to enhanced glycolysis in blastocysts compared with exposure from 2-cell stage. Furthermore, LY-treatment also upregulated oxidative phosphorylation in bovine embryonic stem cells (ESCs). These findings establish RARγ as an important regulator of bovine embryo development, with its inhibition offering a novel strategy to optimize IVF embryo culture systems for livestock production.

ORGANISM(S): Bos taurus

PROVIDER: GSE305071 | GEO | 2025/12/03

REPOSITORIES: GEO

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